1. Post-mortem findings in a patient with avian influenza A (H5N6) virus infection 
R. Gao, M. Pan, X. Li, X. Zou, X. Zhao, T. Li, H. Yang, S. Zou, H. Bo, J. Xu, S. Li, M. Zhang, Z. Li, D. Wang, S.R. Zaki, Y. Shu 
Clinical Microbiology and Infection 
Volume 22, Issue 6, Pages 574.e1-574.e5 (June 2016)
DOI: /j.cmi
Copyright © 2016 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

2. Fig. 1
Histopathological findings in autopsy tissues. The histopathological features were determined by routine haematoxylin & eosin staining. Lung tissue of the patient showed diffused alveolar damage, including intra-alveolar oedema, extensive intra-alveolar haemorrhage, necrosis of alveolar lining cells, focal desquamation of pneumocytes in alveolar spaces, interstitial lymphocytes, phlogocyte and mononuclear inflammatory cell infiltrates (arrow) (a). Pulmonary vasculitis, which presented with oedema, and inflammation in vessel wall was observed in lung tissue (arrow) (b). Trachea presented focal denudation of the epithelium with oedema (arrow) (c), and lymphocyte infiltration was observed in mucosa (arrow) (d). Blood sinus expansion (arrow) and depletion of lymphocytes were observed in lung lymph gland (e). Spleen showed congestion (f). Brain showed oedema of neuroglial cells (five-pointed star) and focal neuronal degeneration in epencephala (g) and brainstem (arrow) (h). Liver presented hepatic spotty necrosis (arrow) accompanied by fatty degeneration in some hepatocytes (i). Lymphocyte infiltration (arrow) or anapetia (five-pointed star) presented in ileum (j) or rectum (k). Focal myocyte injury was observed in heart tissue (arrow) (l). Original magnification: ×20.
Clinical Microbiology and Infection  , 574.e1-574.e5DOI: ( /j.cmi )
Copyright © 2016 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

3. Fig. 2
The influenza nucleoprotein (NP) antigen distribution and viral load in selected autopsy tissues, innate immune cell infiltration and complement activation in lung tissues. Immunohistochemical staining with monoclonal antibody against NP showed positive stains (brown) scattered in pneumocytes of lung tissue (a). Sporadic stains presented in lymphocytes of pulmonary lymphoid gland (b) and spleen (c), epithelia of ileum (d) and rectum (e), hepatocytes (of liver) (f), neuron of epencephala (g) or epithelia of kidney (h). The diagram displayed quantification of the influenza virus matrix gene (log2 of the matrix gene/B2M housekeeping gene) in selected autopsy tissues (i). Highly abundant CD68+ (j) and scattered CD8+ T-cell infiltration (k) in alveolar tissue; cells with activated complement molecular C4d+ were scattered in alveolar (l) and pulmonary vascular (m) tissue; cells with activated complement C5a+ were extensively distributed in alveolar (n), and scattered in pulmonary vascular (o) tissue. Original magnifications: ×40 (a–h, j–l, n); ×20 (o); ×10 (m).
Clinical Microbiology and Infection  , 574.e1-574.e5DOI: ( /j.cmi )
Copyright © 2016 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions