1. CHAPTER 20 DNA TECHNOLOGY AND GENETIC ENGINEERING
PowerPoint® Lecture Slide Presentation by Robert J. Sullivan, Marist College
Human Biology
Concepts and Current Issues
Second Edition
MICHAEL D. JOHNSON
CHAPTER 20 DNA TECHNOLOGY AND GENETIC ENGINEERING
Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings.

2. DNA Sequencing
Figure 20.1

3. DNA Sequencing: DNA Structure
Primers
DNA Polymerase

4. Gel Electrophoresis
Figure 20.2

5. Recombinant DNA Technique
Figure 20.3

6. Laboratory Modified DNA
Recombinant DNA: Cutting, Splicing, Copying DNA
Restriction Enzymes
DNA Ligases
Plasmids

7. Polymerase Chain Reaction (PCR)
Figure 20.4

8. Genetic Engineering: Transgenic Organisms
Transgenic Bacterial Uses
Insulin
Human Growth Hormone
Erythropoeitin
Tissue Plasminogen Activator (tPA)
Vaccines

9. Genetic Engineering: Transgenic Organisms
Transgenic Plants
Increased resistance to freezing
Longer shelf life
Increased Vitamin A
Edible Vaccines
Human Proteins, i.e., albumin

10. Transgenic Plants
Concerns
“Frankenfoods”
Plant disease susceptibility

11. Transgenic Animals Bigger challenges Successes No plasmid use
Cloning more difficult
Successes
Bovine growth hormone for faster animal growth
“gene farming”

12. Genetic Map
Figure

13. Genetic Map
Figure

14. Gene Therapy: Hope of the Future
Obstacles
Transplant into “right” cells
Into reproductive cells?

15. Vectors Transfer Genes to Human Cells
Figure 20.11

16. Vectors Transfer Genes to Human Cells
Retroviruses
Liposomes
Direct DNA injection

17. Gene Therapy Successes
Severe Combined Immunodeficiency (SCID) Success
Cystic Fibrosis
Cancer Research