2. “Proteomics is a science that focuses on the study of proteins: their roles, their structures, their localization, their interactions, and other factors.”

3. The “omics” nomenclature…
Genomics
DNA (Gene)
Functional
Transcriptomics
RNA
Proteomics
PROTEIN
Metabolomics
METABOLITE
Transcription
Translation
Enzymatic
reaction

4. Kinds of Proteomics Functional Proteomics Expressional Proteomics
The identification of protein functions, activities or interactions at a global or organismwide scale.
Expressional Proteomics
The analysis of global or organismwide changes in protein expression.
Structural Proteomics
The high throughput, or high volume expression and structure determination of proteins by Xray, NMR or computer based methods.

5. Components of Expressional Proteomics
Protein Separation
Mass Spectroscopy
Bioinformatics

6. Applications: Biology and disease research
Molecular event description (signaling
molecules, biomarkers)
Finding disease mechanisms and drug
targets

7. Roles of Proteins Proteins are the instruments through which the
genetic potential of an organism are expressed =
active biological agents in cells
Proteins are involved in almost all cellular processes
and fulfill many functions
*enzyme catalysis, transport, mechanical support,
organelle constituents, storage reserves,
metabolic control, protection mechanisms,
toxins, and osmotic pressure

8. Why Proteomics?
Whole Genome Sequence –complete, but does not show how proteins function or biological processes occur
Post-translational modification
*proteins sometimes chemically modified or regulated after synthesis
Proteins fold into specific 3-D structures which determine function
Gain insight into alternative splicing
Aids in genome annotation

10. The Human Proteome Initiative. (2007) http://ca. expasy
The Human Proteome Initiative. (2007) Retrieved March 24, 2009.

11. Work flow and Technologies
Proteomics
Work flow and Technologies
2-dimensional gel based technologies
Mass spectrometry for protein identification and quantification

12. 11/17/2018

13. 2-D Gel Electrophoresis
1st dimension: Isoelectric focusing (IEF)
separate proteins based on their isoelectric point (pI) by using immobilized pH gradient (IPG) gel strips.
2nd dimension: SDS PAGE
separate proteins based on their molecular mass.
run perpendicular to Isoelectric focusing.
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16. 11/17/2018

17. 2-dimensional gel based technologies
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