1. The Alfin-like PHD Zinc Finger Transcription Factor Family
in Arabidopsis Thaliana
Melissa Borkenhagen 8 May 2006
HC70AL
DR. Bob Goldberg

2. Gene AT5G20510 T-DNA 5’ UTR EXON 3’ UTR INTRON Chromosome 5 LBb1 FW
T-DNA INSERT ACCORDING TO SALK
Chromosome 5

3. Gene AT3G42790 T-DNA 5’ UTR EXON 3’ UTR INTRON Chromosome 3 LBb1 FW
T-DNA INSERT ACCORDING TO SALK
Chromosome 3

4. In which plant organs are the genes active?
Gene AT5G20510
Gene AT3G42790
Consistent with GeneChip Data, both
genes appear to be active in these plant organs.

5. Gene AT5G20510 Plant Genotypes
Separation of primers
Only one T-DNA Insert
Plant 1 is the only Homozygous Mutant Plant
Strange double bands
What are the genotypes?

6. Gene AT3G42790 Plant Genotypes
Plants 1 and 4 are Homozygous Mutants
Plant 5: Heterozygous?

7. Are there any observable phenotypic differences between wild type and mutant plants?
No significant differences observed between wild type and mutant plants.

8. Is continued work on this family of transcription factors important?
Research has shown that members of the alfin-like PHD zinc finger transcription factor family may be influential in plant development. Some appear to be particularly influential during seed development, with a possible role in the differential seedling development in darkness and light. Thus, continued work on these proteins is important!