1. Growing High Temperature Microbes in Geochemically Designed Media
Selisa Rollins
Mentor: Dr. Everett Shock
Arizona State University

2. Europa and Mars

3. Why Yellowstone? Water Warm
Easy access to microorganisms that do not use photosynthesis.

4. Hot Spring Samples

5. Geochemical Data Element Amount (kg/mol) Cl- 6.29E-03 Br- 6.98E-06
SO4-2
2.03E-04
PO4-3
3.85E-07
Li+
2.97E04
Na+
1.32E-02 K+
2.64E-04
Ca+2
2.00E-06
pH=7.678
Temperature=79.4°C
Conductivity=2900μS

6. Experimental Design
Representative Geochemical Data
Laboratory

7. Culturing Microbes Inoculate Synthetic
Hot Spring Media with Hot Spring Biomass
Prepare Test Tubes Containing Media
Place Tubes in 80°C Oven

8. Results

9. Summary and Plans Transfer sustained microbes Isolate a microbe
Carbon Source
Observations
Peptone
+++
Yeast Extract
++++++
Peptone and Beef
+++++
Glucose +
Yeast and Peptone
Transfer sustained microbes
Isolate a microbe
Characterize microbe
+ represents # of microbes per screen view

10. Questions?