1. Seminal Fluid Analysis
Lab 9,10
Seminal Fluid Analysis

2. Useful biochemical Test
Fructose:
Fructose is the primary energy source for sperm.
It is required for spermatozoa survival in an anaerobic environment and it stimulates sperm motility.
Spermatozoa, which are subjected to centrifugation and thus separated from the seminal plasma, will not survive anaerobically unless seminal plasma or carbohydrates source is added back to separated spermatozoa.
Seminal plasma fructose is produced by the seminal vesicles. Fructose production is stimulated by testosterone.

3. Since the seminal vesicles do not have a large storage capacity, collection of several ejaculates within a few days will yield decreased fructose values.
It takes about two days for fructose levels in the seminal vesicle to be replenished.
Fructose measurements are useful diagnostically in men with low-volume ejaculates.
The absence of fructose can indicate the congenital absence or infections that affect the seminal will also result in absent or reduced fructose concentration.

4. Reagent Procedure ZnSO4 (5%) Ba(OH)2 (4.76%) Resorcinol (1%) Conc. HCL
Water bath (90oC), automatic pipette ,tubes.
Procedure
In three tubes B, T ,ST we put

5. Then mix, we will show white ppt, leave it 5 min.
Centrifuge at high speed for 5 min.
After that we take from the three tubes 2 ml of supernatant of each, as the table under we do:
Mix, put it in water path at least time 10 min. we will show pink color .
Cool the tubes under tap water, and then read it on 490 nm wave length of spectrophotometer.

6. Calculation : Conc. St= 350mg / dl Abs. T = 0.502 Abs. St = 0.575
Conc. T =( Abs. T / Abs. St )*Cons. St
= 0.502/0.575*350
= mg/dl
50mg / dl

7. Biochemical changes that may be seen in the semen with a Variety of different causes of infertility

8. Serological Analysis Anti-Sperm Antibodies
Antibodies to sperm can be present in the serum of some females as well as males, the seminal fluid and the cervical mucosa are statistically associated with an increased risk of infertility.
It is known that antibodies directed toward various sperm antigens can result in reduced fertility in men.
Pyospermia and the microbiology of semen
Increased number of WBC’s in semen is known as pyospermia and in occasions, is a cause of infertility in men, and so must be examined microbiologically.
The first step in the microbiological examination is to make a Gram stain and then make semen culture.

9. Report interpretation