1. Quiz #7 (8%) Biol710 11/21/12 name___________
What biological process is being mimicked by current DNA sequencing technologies? (2%)
_________________________________________________________________
Why do current DNA sequencing technologies only assign G, A, T, C to DNA sequences
and not Cme, Ame, etc…? (2%)
________________________________________________________________ Q2
You are setting up a two plasmid system to identify an enhancer binding protein.
One plasmid will assess binding, and the other will carry the expressed protein (cDNA).
Where will you put enhancer sequence in the first plasmid below? Draw arrow. (1%)
Is there a benefit to putting more than 1 site (1%)?
_____________________________________________________________
What does the blue box represent in this experiment (1%)?___________________________
(be specific)
minimal promoter (TATA box)
The second plasmid will come from a cDNA library of potential DNA binding proteins.
Be specific: How will you construct the second plasmid to ensure that this two plasmid system
will identify the protein of interest (1%)