1. A 4
Band intensity
(relative unit) 3 2 1
MMP-9
MMP-2 B C 4 4
MMP-9 activity
(relative unit)
MMP-9 activity
(relative unit) 3 3 2 2 1 1
HMGB1 (nM)
HMGB1 (nM)
sRAGE (nM)
rTMD123 (nM)
SUPPLEMENTARY FIGURE 2. rTMD123 attenuates HMGB1-induced MMP-9 in differentiated THP-1 cells. A, activities of MMP-9 and MMP-2 were assessed after HMGB1 treatment for 24 hours (n=4). B, MMP-9 activity was assessed after sRAGE pretreatment for 1 hour and HMGB1 treatment for 24 hours (n=4). C, MMP-9 activity was assessed after rTMD123 pretreatment for 1 hour and HMGB1 treatment for 24 hours (n=4). (All activities were assessed by gelatin zymography. *P<0.05, **P<0.01, n.s. P>0.05 compared with untreated group. #P<0.05 compared with HMGB1-treated group.)