1. RECOMBINANT DNA TECHNOLOGY
PART 2

2. Topics Genomic libraries
Id a specific clone or sequence within a library
Transgenic plants
Transgenic animals
Timely topics

3. Genomic library contains all of the organism’s genome
Probe design

4. Bacterial clones and finding desired clone
Probe design

5. A Final about vectors : There are many types 
Small vectors
Large vectors
Expression vectors
Viral vectors

6. Transgenic Mice

7. transgene
Non-homologous recombination
Linear DNA

8. Knockout Mice

9. G418 gancyclovir Homozygous knockout
Phenotype of the knockout mouse gives information on protein function

10. Applications of DNA technology
Agriculture
Drug design
Genetic testing
Gene therapy

11. Genetic Modified Plants: “GM”
Tobacco with luciferase gene from firefly
Transgenic plant

12. Tumor Inducing Plasmid

13. Model for making genetically modified (GM) plants

14. GM crops
Bt toxin genes (Bacillus thuringensis)

15. Bt Toxin
Cry toxins and cry genes: damages gut of larvae

16. GM Plants Roundup  ready plants: herbicide resistant
Herbicide known as: Glyphosate

17. Glyphosate Enzyme inhibitor Prevents amino acid synthesis
Prevents weed growth
Environmental concerns

18. Antisense Technology
5´   C A U G   3´     mRNA 3´   G U A C   5´     Antisense RNA

19. Anti-Sense Technology & FLAVR SAVRTM Tomato
Anti-sense DNA technology inhibits polygalacturonase (PG)

20. The Flavr-Savr Tomato Ripens on the vine Preserves firmness and flavor
Uses anti-sense technology

21. Cloning of a whole organism: Reproductive Cloning (you can read about this on page 601 of your book as well)

22. SHEEP CELLS 54 27
Somatic Cell Germ Cell
For ex. Mammary cell ovum

23. How to process begins: 54 27 54 discarded Somatic Cell Germ Cell
For ex. Mammary cell ovum

24. Now the germ cell contains 54 chromosomes!
The germ is stimulated “electrically”.
Cell division in some instances begins.
Individual is identical to host “DNA” donor.
Aging affects?
“Was Dolly a sheep in lamb’s clothing?”

25. Somatic Gene Therapy

26. Successes SCIDS (Severe Combined Immunodeficiency Disease)
Head and neck cancers

27. But also failures and controversy