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Published byHorace Holt Modified over 6 years ago
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Module 4 Preparation of solid media for culture and DST
Exercise
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Good- or poor-quality media?
Different dye bubbles
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Explain possible reasons for poor quality of media.
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Answers Bubbles (problems in the coagulation process).
Non-homogeneous dye (non-homogeneous melting). Discoloured (problems in the coagulation process). Contamination (problems in sterilization and/or during the procedure). ≥8 weeks old (expired, may give false-negative results). Not properly stored (may give false-negative results).
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Scenario 1 You have prepared a batch of LJ medium. After 24 hours of incubation at 37 °C, colonies have appeared on the surface of the medium. Find out possible causes and propose solutions.
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Scenario 2 You have prepared a batch of plain medium. After coagulation, the medium remains soft/liquid. Find out possible reasons and propose remedies.
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Scenario 3 You have prepared a batch of plain medium. After coagulation, the medium has bubbles. Find out possible reasons and propose remedies.
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Scenario 4 You have prepared a batch of plain medium. After coagulation, the medium has a non- homogeneous dye. Find out possible reasons and propose remedies.
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