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UD. IV. GENÈTICA. Ll. IV. 5. Biotecnologia

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Presentation on theme: "UD. IV. GENÈTICA. Ll. IV. 5. Biotecnologia"— Presentation transcript:

1 UD. IV. GENÈTICA. Ll. IV. 5. Biotecnologia
4. Estudi de la seqüència d’ADN. Mètode didesoxi.

2

3 Figure 20.12 TECHNIQUE DNA (template strand) Primer Deoxyribonucleotides Dideoxyribonucleotides (fluorescently tagged) T 3 5 G C T dATP ddATP T T 5 G dCTP ddCTP A C DNA polymerase dTTP ddTTP T T dGTP ddGTP C G A P P P P P P C G G A 3 A OH H DNA (template strand) Labeled strands 5 3 C ddG T ddA A G ddC C C A ddT T T T C ddG G G G G T ddA A A A A A T ddA A A A A A A C ddG G G G G G G G G ddC C C C C C C C C A T T T T T T T T T T T T T T T T T C G G G G G G G G G A T T T T T T T T T 3 A T T T T T T T T T 5 Shortest Longest Direction of movement of strands Longest labeled strand Figure Research Method: Dideoxy Chain Termination Method for Sequencing DNA Detector Laser Shortest labeled strand RESULTS Last nucleotide of longest labeled strand G A C T G Last nucleotide of shortest labeled strand A A G C

4 Deoxyribonucleotides Dideoxyribonucleotides (fluorescently tagged)
Figure 20.12a TECHNIQUE DNA (template strand) Primer Deoxyribonucleotides Dideoxyribonucleotides (fluorescently tagged) T 3 G 5 C T dATP ddATP T T 5 G dCTP ddCTP A C DNA polymerase dTTP ddTTP T T dGTP ddGTP C G A P P P P P P G C G Figure Research Method: Dideoxy Chain Termination Method for Sequencing DNA A A OH H 3

5 didesoxinucleòtid

6 Direction of movement of strands Longest labeled strand
Figure 20.12b TECHNIQUE (continued) DNA (template strand) Labeled strands 3 5 C ddG T ddA A G ddC C C A ddT T T T C ddG G G G G T A A A A A ddA T ddA A A A A A A C ddG G G G G G G G G ddC C C C C C C C C A T T T T T T T T T C G G G G G G G G G A T T T T T T T T T 3 A T T T T T T T T T 5 Shortest Longest Direction of movement of strands Longest labeled strand Figure Research Method: Dideoxy Chain Termination Method for Sequencing DNA Detector Laser Shortest labeled strand

7 Direction of movement of strands Longest labeled strand
Figure 20.12c Direction of movement of strands Longest labeled strand Detector Laser Shortest labeled strand RESULTS Last nucleotide of longest labeled strand G A C Figure Research Method: Dideoxy Chain Termination Method for Sequencing DNA T G A Last nucleotide of shortest labeled strand A G C

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