1. Assessment of peripheral skeletal muscle microperfusion in a porcine model of peripheral arterial stenosis by steady-state contrast-enhanced ultrasound and Doppler flow measurement 
Claas P. Naehle, MD, Verena A. Steinberg, MD, Hans Schild, MD, Gottfried Mommertz, MD 
Journal of Vascular Surgery 
Volume 61, Issue 5, Pages (May 2015)
DOI: /j.jvs
Copyright © 2015 Society for Vascular Surgery Terms and Conditions

2. Fig 1 Experimental protocol. CEUS, Contrast-enhanced ultrasound.
Journal of Vascular Surgery  , DOI: ( /j.jvs )
Copyright © 2015 Society for Vascular Surgery Terms and Conditions

3. Fig 2
Stenosis creation. A 5-mm-wide graft (open arrow) is wrapped around the external iliac artery (black arrow) and then gradually tightened with sutures through the graft (open arrow) until the target pressure gradient is reached.
Journal of Vascular Surgery  , DOI: ( /j.jvs )
Copyright © 2015 Society for Vascular Surgery Terms and Conditions

4. Fig 3
Box and whisker plots of all contrast-enhanced ultrasound (CEUS) and Doppler flow measurements performed at rest and at stress without stenosis and after creation of a moderate stenosis. A, Mean transit time (mTT). B, Wash-in rate (WiR). C, Perfusion index (PI). D, Relative blood volume (rBV). E, Doppler flow. *Statistical significance. a.u., Arbitrary units.
Journal of Vascular Surgery  , DOI: ( /j.jvs )
Copyright © 2015 Society for Vascular Surgery Terms and Conditions

5. Fig 4
Example of contrast-enhanced ultrasound (CEUS) perfusion quantification: top, B-mode ultrasound and CEUS images; below, curve of ultrasound signal intensity over time; bottom, mean transit time (mTT) measured for region of interest as drawn in CEUS image. A, Rest perfusion without stenosis. B, Rest perfusion at the same location after creation of a moderate stenosis.
Journal of Vascular Surgery  , DOI: ( /j.jvs )
Copyright © 2015 Society for Vascular Surgery Terms and Conditions