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Published byTeresa Valenzuela Salas Modified over 6 years ago
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Does Gene AT5G19490 Play a vital role in seed development?
Research Presentation By Ben Torres HC70AL Spring 2004 AT5G19490 FW >>> RV <<<< 1 1073bp
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Did Molecular biology techniques facilitate our quest for knowledge?
FW+Jl202 How can we unambiguously tell if there is a T-DNA insert in Gene AT5G19490? RV+Jl202 AT5G19490 Why didn’t the experiment end by picking the super pool with fragments smaller than the gene AT5G19490 on the gel?
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What did the AutoRadiograph reveal
How was the size of the fragments known? AT5G19490 900bp fragment Did this show unambiguously the smaller fragment was a T-DNA insert?
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What do you do When all else fails?
cDNA of ortholog gene ATG19490 in the scarlet runner bean SIGnAL "T-DNA Express" Arabidopsis Gene Mapping Tool
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Why not do conduct a microarray on the arabidopsis itself?
Key for corresponding Stages of plant life On the X axis. 1=wild type ovules 2=wild type 24-hour seeds 3=wild type mid-maturation (Cotyledon stage) 4=wild type mature green seeds 5=wild type post maturation green seeds 6=wild type seedlings (3DAI) 7=wild type leaves 8=wild type roots 9=wild type stem 10=wild type C24 leaves
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What about the Salk lines
Possible T-DNA homozygous Salk lines Does this show the full genotype? Wild type PCR conducted on salk lines How did the rest of the Genotyping go?
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What about the Madison DNA pools
PossibleT-DNA insert
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What next? Genotype Salk lines using the FW primer ant the LBb1 T-DNA primer correctly to unambiguously define the genotypes Try to obtain a correct sequence from the DNA pool containing the fragment size of interest.
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