1. Nor + + - - - Supp.Fig. S1 Raja et al. a b c d e f 1 2 Hyp - + Hyp - +
OPN
Hyp
Time (hr)
Actin
MCF-7 b
OPN/
DAPI
Hyp
Time (hr) 1 2
MCF-7
HIF1α
HIF1β
Actin
Nor
Hyp
Coni
OPNi
β3i
MCF-7 c
Actin d
β3-Integrin
β3i C
Coni
OPN
C Coni OPNi
MDA-MB-231 e
GAPDH (188 bp)
OPN (400 bp)
WT KO
MEF
200 bp
400 bp
300 bp
Actin
HIF1α
MCF-7
Hyp
rhOPN
(μM) f
Supp.Fig. S1 Raja et al.

2. Supp.Fig. S2 Raja et al. a b c Akt 1 2 3 p-p65 p65 p-Akt 231 OPNshRNA
p-p65
p65
p-Akt
231
OPNshRNA
Nor
Hyp
MDA-MB-231 b
Actin
HIF1α
VEGF
231
OPNshRNA
Nor
Hyp
MDA-MB-231 c
Actin
OPN
OPNshRNA
MDA-MB-231
Supp.Fig. S2 Raja et al.

3. Supp.Fig.S3 Raja et al. a b c * * Nor Hyp Hyp+ Coni Hyp + OPNi
T=0 h
T=16 h
Nor Hyp Hyp+ Coni Hyp + OPNi
Hyp + β3 i Hyp +IgG Hyp +OPN Neu Ab
MDA-MB-231 a
% Wound Closure c
MDA-MB-231
T=0 h
T=16 h
Hypoxia
rhOPN (0.25 µM)
OPN shRNA b * *
Hyp
rh OPN
(0.25 µM)
OPN shRNA
Supp.Fig.S3 Raja et al.

4. Supp.Fig.S4 Raja et al. Nor T=0h T=16h Hyp OPN (0.5μM) - + + + -
Coni
HIF1α i
T=0h
T=16h
Nor
Hyp
MDA-MB-231
Supp.Fig.S4 Raja et al.

5. Supp.Fig.S5 Raja et al. a c b * ** Coni Coni OPNi β3 i MDA-MB-231 Nor
Hyp
Migration c
Nor Hyp Hyp+ Coni
Hyp +OPNi Hyp+ β3 i
MDA-MB-231
% Migration
Nor
Hyp
Coni
OPNi
β3 i * ** b
Invasion
Supp.Fig.S5 Raja et al.

6. MCF-7
MCF-OPN-a
HIF2α
DAPI
Hyp
(16 h)
Supp.Fig.S6 Raja et al.

7. Supplementary Table S1 Raja et al.
Correlation coefficient of OPN and HIF1α expression in human breast cancer
Supplementary Table S1 Raja et al.

8. ** Correlation is significant at 0.01level (2 tailed)
Correlation coefficient of OPN, HIF1α and HIF2α expression in human breast cancer
Spearman’s rho
Correlation Coefficient
Sig (2-tailed)
HIF1α -OPN
0.613
P=0.005**
HIF1α - HIF2α
0.181
P=0.458
OPN- HIF2α
0.337
P=0.158
N=19
** Correlation is significant at 0.01level (2 tailed)
Supplementary Table S2 Raja et al.