1. Introduction to Enzyme Function Lab

2. Period 2 Results
Look at your results. In which test tubes did you observe bubbles or balloon inflation?

3. Period 2 Results (Glucose Control)
Why were the balloons on the glucose control test tubes consistently inflated?

4. Period 2 Results (Untreated Lactase)
What was in the untreated lactase test tubes?
What would you expect to happen in this test tube?
How does that compare to what we actually saw?
What could this tell us about the enzyme?

5. Period 2 Results (Temperature)
Test tube 5 with the inflated balloon corresponds to the lactase enzyme treated at 40°C. What is 40°C in degrees Fahrenheit?
Why might this temperature have worked and the others failed?
How does temperature affect the lactase and lactose in this test tube?

6. Period 2 Results (pH)
pH pH pH 8

7. Lab Summary
What evidence from your experiment indicated whether lactase was functioning?
What were the controls in the experiment? What information did they provide?

8. What evidence from you experiment indicated whether lactase was functioning?

9. Overall Results
I conducted the temperature portion of the experiment on my own and got these results.
What does each test tube tell us?
Why did we need so many controls?

10. Overall Results Balloon Number 1 (Yeast) 2 (Glucose control)
3 (Lactose control)
4 (Lactase control)
5 (40°C)
6 (60°C)
7 (80°C)
8 (100°C)
(Untreated lactase)
Observation
none
yes
some
Balloon Number
1 (Yeast) 2
(Glucose control)
3 (Lactose control)
4 (Lactase control)
5 (pH 4)
6 (pH 6)
7 (pH 8)
(pH 10)
(Untreated lactase)
Observation
none
yes
some

11. Period 5 Results
Look at your results. In which test tubes did you observe bubbles or balloon inflation?

12. Period 5 Results (Glucose Control)
Why were the balloons on the glucose control test tubes consistently inflated?

13. Period 5 Results (Untreated Lactase)
What was in the untreated lactase test tubes?
What would you expect to happen in this test tube?
How does that compare to what we actually saw?
What could this tell us about the enzyme?

14. Period 5 Results (pH)

15. Period 2 Results (pH)
pH pH pH 8

16. Overall pH Results
The pH results for Period 5 consistently showed balloon inflation in all four test tubes.
Do those results make sense?
Why do you think this happened?
What would you expect the results to be and why?

17. Period 5 Results (Temperature)
This image shows a small amount of balloon inflation in each of the temperature-treated test tubes.
Did anyone notice if one particular temperature was inflating more than another?

18. Period 2 Results (Temperature)
Test tube 5 with the inflated balloon corresponds to the lactase enzyme treated at 40°C. What is 40°C in degrees Fahrenheit?
Why might this temperature have worked and the others failed?
How does temperature affect the lactase and lactose in this test tube?

19. Lab Summary
What evidence from your experiment indicated whether lactase was functioning?
What were the controls in the experiment? What information did they provide?

20. What evidence from you experiment indicated whether lactase was functioning?

21. Overall Results
I conducted the temperature portion of the experiment on my own and got these results.
What does each test tube tell us?
Why did we need so many controls?

22. Overall Results Balloon Number 1 (Yeast) 2 (Glucose control)
3 (Lactose control)
4 (Lactase control)
5 (40°C)
6 (60°C)
7 (80°C)
8 (100°C)
(Untreated lactase)
Observation
Balloon Number
1 (Yeast) 2
(Glucose control)
3 (Lactose control)
4 (Lactase control)
5 (pH 4)
6 (pH 6)
7 (pH 8)
(pH 10)
(Untreated lactase)
Observation