1. SERUM CONTAINING MEDIA AND ITS COMPONENTS

2. INTRODUCTION
MEANING OF THE TERMS: 1.MEDIA 2.SERUM

3. MEDIA
Two types: *Natural Media *Artificial Media

4. NATURAL MEDIA Extracted from biological resources. CLOTS: Plasma clots
NATURAL MEDIA Extracted from biological resources *CLOTS: Plasma clots *BIOLOGICAL FLUID: Amniotic fluid. Pleural fluid.aqueous humour from eye,serum *TISSUE EXTRACT: Extract from lymph,spleen,bone marrow,chick embryo extract

5. ARTIFICIAL MEDIA It is grouped into four classes
ARTIFICIAL MEDIA It is grouped into four classes *Serum containing media *Serum free media *Chemically defined media *Protein free media

6. COMPOSITIONS PROTEINS: 1.FIBRONECTIN: *Attachment *spreading factors 2.TRANSFERIN: binding protein

7. GROWTH FACTORS: 1. PGDF: Platelets Derived Growth Factor 2
GROWTH FACTORS: 1.PGDF: Platelets Derived Growth Factor 2.EGF: Epithelial Growth factor 3.FGF:Fibroblast Growth Factor 4.IGF: Insulin like Growth Factor

8. GROWTH HORMONES Insulin promotes the uptake of glucose n amino acids Growth hormones may be present in serum particularly foetal serum

9. SERUM MEDIA ADVANTAGEs 1. complete media 2. Increases the viscosity 3
SERUM MEDIA ADVANTAGEs 1. complete media 2. Increases the viscosity 3. It acts as a buffher 4. no charged substrate is required 4.

10. DISADVANTAGES 1. Serum contains growth inhibitor components 2
DISADVANTAGES 1.Serum contains growth inhibitor components 2.targe variation in serum quality from one batch to other 3.it enhances the chances of contamination 4.Contains cytotoxic components.