1. Therapeutic angiogenesis with intramyocardial administration of basic fibroblast growth factor 
Michio Kawasuji, MD, Hiroshi Nagamine, MD, Masahiro Ikeda, MD, Naoki Sakakibara, MD, Hirofumi Takemura, MD, Susumu Fujii, MD, Yoh Watanabe, MD 
The Annals of Thoracic Surgery 
Volume 69, Issue 4, Pages (April 2000)
DOI: /S (99)01557-X

2. Fig 1
Experimental model used to make myocardial infarction by ligation of the left anterior descending coronary artery (LAD) and provide direct administration of basic fibroblast growth factor or placebo into the myocardium. The left atrial catheter was positioned for administration of colored microspheres. After all blood flow measurements, the heart was cut into short-axis slices and the left ventricle was divided into eight segments. (LCX = left circumflex coronary artery; RCA = right coronary artery.)
The Annals of Thoracic Surgery  , DOI: ( /S (99)01557-X)

3. Fig 2
Heart tissue in the border zone of infarct in a dog treated with basic fibroblast growth factor (bFGF) (A) and a control dog (B). Vessels were expressed by staining endothelial cells using a monoclonal antibody against von Willebrand factor. Increased capillaries and arterioles were noted in an infarct-adjacent region of a bFGF-treated dog. (×200.)
The Annals of Thoracic Surgery  , DOI: ( /S (99)01557-X)

4. Fig 3
Effect of basic fibroblast growth factor (bFGF) on myocardial blood flow, which is shown as percent of normal (%MBF). A marked increase in %MBF is evident in bFGF-treated dogs during the period from 3 to 7 days after infarction. (A) Endocardial portion of the infarcted area. (B) Epicardial portion of the infarcted area. (C) Endocardial portion of the border zone. (D) Epicardial portion of the border zone. (NS = not significant.)
The Annals of Thoracic Surgery  , DOI: ( /S (99)01557-X)

5. Fig 4
Effect of basic fibroblast growth factor (bFGF) on left ventricular function. Treatment with bFGF improved left ventricular ejection fraction 7 days after infarction. (LVEDV = left ventricular end-diastolic volume; LVESV = left ventricular end-systolic volume; NS = not significant.)
The Annals of Thoracic Surgery  , DOI: ( /S (99)01557-X)

6. Fig 5
Numerical density of capillaries and arterioles in the border zone of infarct, expressed as vessels per visual field. Treatment with basic fibroblast growth factor (bFGF) increased the number of capillaries and arterioles.
The Annals of Thoracic Surgery  , DOI: ( /S (99)01557-X)