1. Dieter Steinmetz Universität Tübingen – ZMBP 18.12.15 Kurs WS 15/16
Gene Editing
Dieter Steinmetz
Universität Tübingen – ZMBP Kurs WS 15/16

2. Gene Editing – 3 Main Methods
ZFN „Zinc Finger Nuclease“ TALEN “Transcription Activator-Like Effector Nuclease” CRISPR/Cas9 nuclease “Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 nuclease”
ZMBP - Dieter Steinmetz
Quelle: 1. Baltes & Voitas und 3. Wikimedia Commons

3. ZFN „Zinc Finger Nuclease“
ZMBP - Dieter Steinmetz

4. ZFN – TALEN – CRISPR – gleicher Mechanismus
Suchsequenz DSB Disruption oder Substitution
Chen et al. GigaScience :24   doi: / X-3-24
ZMBP

5. Gene targeting effektiver in CRISPR
Gene targeting Experimente
Merkle FT et al. (2015). Efficient CRISPR-Cas9-Mediated Generation of Knockin Human Pluripotent Stem Cells Lacking Undesired Mutations at the Targeted Locus. Cell Reports. Volume 11, 6, 12 May 2015, Pages 875–883
ZMBP

6. Protein B
Protein A

7. Recent advances enable precision in editing
Random Mutagenesis & Integration RNAi Mediated Gene Knockout Targeted Integration via Homologous Recombination First Generation Genome Editing Tool Zinc Finger Technology

9. Recent advances enable precision in editing
Random Mutagenesis & Integration
RNAi Mediated Gene Knockout
Targeted Integration via Homologous Recombination
First Generation Genome Editing Tool Zinc Finger Technology

10. Random Mutagenesis & Integration RNAi Mediated Gene Knockout
Targeted Integration via Homologous Recombination
First Generation Genome Editing Tool Zinc Finger Technology