1. Supplement 1 -Psen1 -Psen2 Dasatinib (100 nM) Hours Con 24 48 72
-cyclin D1
-Psen1
-Psen2
- Trop2- ICD
-Vinculin

2. A B C Supplement 2 -Psen1 -Psen2 DMSO Canertinib 200 m 24 h 48 h40 80
120
DMSO
Canertinib
Canertinib
Canertinib
Canertinib
DMSO
DMSO
DMSO
Cell number per 104 m2
-cyclin D1
-Psen1
-Psen2
- Trop2- ICD
-Vinculin

3. Supplement 3 Trop2 TACE ECD γ-Secretase ICD PS1 PS2 Numb Nucleus
Src
Cyclin D1
Stem Cell
Population
Numb
γ-Secretase
PS1
PS2
Nucleus

4. Human prostate cancer TMA IHC
Supplement 4
Human prostate cancer TMA IHC
High expression
Low expression
Cyclin D1
Trop-ICD
Presenilin 2
Numb