1. by Mawadda Alnaeeli, and Yen-Tung A. Teng
Dendritic cells differentiate into osteoclasts in bone marrow microenvironment in vivo
by Mawadda Alnaeeli, and Yen-Tung A. Teng
Blood
Volume 113(1):
January 1, 2009
©2009 by American Society of Hematology

2. Immature but not mature CD11c+ DCs carry the osteoclastogenic potential.
Immature but not mature CD11c+ DCs carry the osteoclastogenic potential. Freshly purified CD11c+ DCs (immature) or bacteria Aggregatibactor actinomycetemcomitans (Aa)–treated DCs for 24 or 48 hours (mature) were cultured with 30 to 100 ng/mL soluble receptor activator nb NFKB ligand (sRANKL) in the presence of 10 μg/mL Aa sonicated Ag (see Alnaeeli4). On day 5, the total number of TRAP+ multinucleated DC-derived OCs developed per unit area were quantified, using the protocol reported previously4 where the results are shown as mean plus or minus SE with *P = .001 (N = 5 experiments), and DC-only and DC + Aa as negative controls and Splenocytes + ConA as positive control.
Mawadda Alnaeeli, and Yen-Tung A. Teng Blood 2009;113:
©2009 by American Society of Hematology