1. Dr. Saif Aldeen AlRyalat
CRISPR-Cas9 system; the new tool to design our own bio-production system
Dr. Saif Aldeen AlRyalat

2. What is CRISPR -Cas9 system?
Clusters of Regularly Interspaced Short Palindromic Repeats
Makarova K, 2011

3. CRISPR-Cas9 in genetic engineering
Easy to use, precise in its cut.
Repair by either NHEJ or HDR
Grammatikakis Y, 2015

4. General Applications 1- Biological 2- Biotechnological 3- Medicine
Hsu P, 2014

5. 400 million worth genetic engineering
Gerngross & Hutchinson
Full glycosylation of erythropoietin via a yeast “Pichia pastoris”

6. CRISPR-Cas9 in humans Chinese trial against lung cancer
US trial on several types of cancers

7. Even Bill Gates! 75 million investment in CRISPR- Cas9.
Wiping out mosquitoes that transmits malaria.
Hammond A, 2016

8. What about embryos? It is the core of ethical debate
It is still beyond our knowledge

9. CRISPR-Cas9 dispute!
University of California VS Massachusetts Institute of Technology
Nobel prize committee is waiting to determine Noble winner.

10. Thank You