1. Augmented epithelial multidrug resistance–associated protein 4 expression in peritoneal endometriosis: regulation by lipoxin A4 
Ilaria Gori, Ph.D., Yoima Rodriguez, M.Sc., Chiara Pellegrini, M.Sc., Chahin Achtari, M.D., Daniela Hornung, M.D., Ph.D., Eric Chardonnens, M.D., Dorothea Wunder, M.D., Maryse Fiche, M.D., Geraldine O. Canny, Ph.D. 
Fertility and Sterility 
Volume 99, Issue 7, Pages e2 (June 2013)
DOI: /j.fertnstert
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Prostaglandin (PG) E2 and its transporter multidrug resistance–associated protein 4 (MRP4) are up-regulated in patients with endometriosis in comparison to control subjects. (A) Peritoneal fluid from endometriosis patients and control subjects were collected and PGE2 measured with the use of a PGE2 EIA kit. Data represent the mean ± SEM. **P<.001 vs. control subjects. (B) MRP4 mRNA expression in the endometrial tissue of control subjects and in eutopic and ectopic endometrium of endometriosis patients was quantified by quantitative reverse-transcription polymerase chain reaction (qRT-PCR) and normalized to GAPDH. Data represent the mean ± SEM. *P<.05 vs. eutopic endometrium. (C) Immunohistochemical localization of MRP4 in endometrial tissue of control subjects (ctrl; a, d, g) and in eutopic (b, e, h) and ectopic (c, f, i) endometrium. Insets (c, f): ×40 magnification. Two representative pictures from each group are shown, with the negative control in the bottom panel.
Fertility and Sterility  , e2DOI: ( /j.fertnstert )
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
Lipoxin A4 (LXA4) reduces MRP4 mRNA and protein levels in endometriotic epithelial cells. 12Z cells were incubated with vehicle or LXA4 at the indicated concentration for 6 hours. (A) Total RNA was extracted and qRT-PCR performed. Data represent the mean ± SEM from six independent experiments. (B) Cell lysates were then analyzed for MRP4 protein levels by Western blot. One representative blot of three is shown. ImageJ software was used for densitometric analysis. Data represent the mean ± SEM. *P<.05; **P< .001; ***P<.0001 vs. vehicle-treated cells. Abbreviations as in Figure 1.
Fertility and Sterility  , e2DOI: ( /j.fertnstert )
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
LXA4-mediated inhibition of MRP4 expression in 12Z cells occurs via estrogen receptor (ER) α. (A) 12Z cells were treated with 100 nM LXA4 for 6 hours and, where indicated, cells were preincubated with ER antagonist ICI for 12 hours or formyl peptide receptor 2/Lipoxin A4 receptor (FPR2/ALX) antagonist Boc2 for 30 minutes. MRP4 mRNA levels were measured by qRT-PCR. Data represent the mean ± SEM from six independent experiments. *P<.05; ***P<.0001 vs. vehicle-treated cells; τP<.05 vs. condition without inhibitor treatment. (B) 12Z cells were transfected with scrambled small interfering RNA (ctrl siRNA) or siRNA targeted against ERα (ERα siRNA) or FPR2/ALX (FPR2 siRNA) for 24 hours. Cells were then incubated with vehicle or 100 nM LXA4 for 6 hours. Total RNA was extracted and subjected to qRT-PCR analysis. Data represent the mean ± SEM from three independent experiments. **P<.001 vs. vehicle-treated ctrl siRNA–transfected cells; τP<.05 vs. condition with ctrl siRNA. (C) Lysates from cells transfected with scrambled siRNA (ctrl siRNA) or siRNA targeted against ERα or FPR2/ALX for 24 hours were made and Western blotting performed. One representative blot of three is shown. Densitometric analysis was carried out with the use of ImageJ software. Data represent the mean ± SEM. ***P<.0001 vs. ctrl siRNA transfected cells. Abbreviations as in Figures 1 and 2.
Fertility and Sterility  , e2DOI: ( /j.fertnstert )
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

5. Figure 4
LXA4 attenuates basal PGE2 release by endometriotic epithelial cells. 12Z cells were incubated with 100 nM LXA4 or vehicle for 6 h. Supernatants were then collected and PGE2 content quantified with the use of an EIA kit according to the manufacturer's instructions. Mean ± SEM from two independent experiments is shown. *P<.05 vs. vehicle-treated cells (ctrl). Abbreviations as in Figures 1 and 2.
Fertility and Sterility  , e2DOI: ( /j.fertnstert )
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions