1. 1 Usage of REST-GCA Zang Zhenxiang School of Science, Beijing jiaotong university State Key Laboratory of Cognitive Neuroscience and Learning, Beijing normal university zangzx416@sina.com

2. 2 Outline Set data directory Set data directory Set ROI Set ROI Mask selection Mask selection Set order Set order Set covariates (optional) Set covariates (optional) Set output directory Set output directory

3. 3 Set directory Click the right button to add a directory: Click the right button to add a directory:

4. 4 Set directory Add sub-folders Add sub-folders for batch analysis: for batch analysis:

5. 5 Set directory Remove the selected directory: Remove the selected directory:

6. 6 Set directory Remove all the directories: Remove all the directories:

7. 7 Set ROI Voxel-wise ROI-wise

8. 8 Voxel-wise

9. 9 Voxel-wise Please see REST-sliceviewer for details. The text file must contain only one column. Each line represents the value of a time point.

10. 10 Set ROI Voxel-wise ROI-wise

11. 11 ROI-wise (between two ROIs) Set the first ROI: Set the first ROI:

12. 12 ROI-wise Please see REST-sliceviewer for details. The text file must contain only one column. Each line represents the value of a time point.

13. 13 ROI-wise (between two ROIs) Set the second ROI: Set the second ROI:

14. 14 ROI-wise Please see REST-sliceviewer for details. The text file must contain only one column. Each line represents the value of a time point.

15. 15 Mask selection

16. 16 User defined mask Select your Select your mask: mask:

17. 17 Order and algorithm Set order: Set order: Choose the algorithm: Choose the algorithm: or

18. 18 Add covariates Covariates are optional Covariates are optional The covariates text file may contain several columns, each respresenting a covariate.

19. 19 Set output directory

20. 20 Click Compute to start

21. 21 Acknowledgment Many thanks to: Dong Zhangye Yan Chaogan Huang Jian Zang Yufeng And all the group members!

22. 22 Thanks for using REST and REST-GCA! Thanks for using REST and REST-GCA!