1. ELISA

2. ELISA

3. Colorimetric Detection

5. Arthropods Enormously diverse phylum
Many parasitic arthropods serve as hosts for a number of parasites (hyperparasitism) from cestodes to viruses
We focus on the crustaceans, insects, and arachnida
Chitinous cuticle that serve as an exoskeleton
Sexes are separate and typically sexual dimorphism is present
Development: egg  larval or nymphal development adult
Larval: stage where major morphological changes occur and nymph refers to stages where sizes change between molts
Taxonomy is constantly changing

6. Important Arthropods Domain: Eukarya Kingdom: Animalia
Phylum: Arthropoda
Class: Insecta
Order: Phthiraptera
Suborder: Anuplura
Family: Pthiridae
Genus and species: Phthirus pubis

7. Phthirus pubis “Crab Lice”
Pubic lice is more common in teenagers
Often spread through sexual contact mm
Exclusively consume blood
Round body and the back pairs of legs are much thicker than the front legs

9. Pediculus humanus
Pediculus humanus humanus (corporis): Body louse (typhus and trench fever)
Pediculus humanus capitis: Head lice
These sucking lice are ectoparasite of mammals and possess a small fused throax and five segmented antennae. They have specialized mouth parts for penetrating the skin
Compound eyes, the mouthparts and the antennae. The thorax bears three pairs of legs and each terminates in a claw

10. Head Lice

11. Sarcoptes scabiei Kingdom-Animalia Phylum-Arthropoda Class-Arachnida
Subclass-Acari
Family-Sarcoptidae
Genus and Species- Sarcoptes scabiei, variation hominis

12. Sarcoptes scabiei
Burrows into your skin and causes scabies. Gravid females cause the most misery as they burrow just below the surface of the skin laying eggs as they proceed. These tunnels then ooze serum which hardens into scabs
The mites move underneath your skin and cause intense itching (direct life cycle)
Relatively small < 1mm
Covered in long hairs.

13. Sarcoptes scabiei

14. Dermacenter andersoni
Kingdom:
Animalia
Phylum:
Arthropoda
Class:
Arachnida
Subclass:
Acari
Superorder:
Parasitiformes
Order:
Ixodida
Family:
Ixodidae
Genus:
Dermacentor
Species:
D. andersoni
Diagnostic features: Larva only has 3 pairs of legs. Nymph has 4 pairs. Single pair of spiracular openings (stigmata) close to the coxae (leg bases or segments) of the 4th pair of legs (except in larvae) Terminal capitulum (mouthparts) visible from above in all stages except the egg.
It looks similar to Ixodes (which caues lymes disease)

15. Dermacenter andersoni
Transmits Rocky Mountain Spotted Fever
Rocky Mountain wood tick

16. Argulus spp. Kingdom: Animalia Phylum: Arthropoda Subphylum: Crustacea
Class:
Maxillopoda
Subclass:
Branchiura
Order:
Arguloida
Family:
Argulidae
Genus:
Argulus
Species:
A. foliaceus

17. Argulus spp. “Fish Lice” Marine, Freshwater, and brackish
One of the most widespread ectoparasites of fish
True compound eyes, a sucking proboscis, modified maxillae that form a pair of sucking cups
The proboscis is then buried in thee skin of the fish to extract blood
Life cycle:
Males and females swim freely and the females need to leave their hosts to lay eggs. Development from the nauplius to the metanauplius to the copepodid occurs within the egg. Copepodids then hatch from the eggs and search for a host

18. Culicoides “biting midges”
African horse sickness
Bovine ephemeral fever
Onchcerca gibsoni
Kingdom:
Animalia
Phylum:
Arthropoda
Class:
Insecta
Order:
Diptera
Family:
Ceratopogonidae
Subfamily:
Ceratopogoninae
Tribe:
Culicoidini
Genus:
Culicoides

19. …..the Botfly….. Dermatobia hominis
Kingdom:
Animalia
Phylum:
Arthropoda
Class:
Insecta
Order:
Diptera
Section:
Schizophora
Subsection:
Calyptratae
Superfamily:
Oestroidea
Family:
Oestridae

20. Cimex lectularius Bed bugs
Skin rashes and can cause psychological effects
May cause serve allergies