1. FANCC Antibody Test 1 2 3 4 1 2 3 4 Conclusions:
Lane 1: FANCC-Flag 293 EBNA lys.
Lane 2: 293 EBNA (-)
Lane 3: FANCC-Flag 293 EBNA lys.
Lane 4: 293 EBNA (-) 72 
FANCC
FANCC  55
Conclusions:
C-2 is better than C-1, but both work
C-2 may detect endogenous in 293 cells
NOTE: C-1 has major cross reacting band near FANCC
1º ab
FANCC-1
1º ab
FANCC-2
1º ab Flag
2º ab anti-rabbit-HRP
2º ab anti-rabbit-HRP
Alexis LaChapelle,
Hoatlin Lab

2. FANCD2 Antibody Test 1 2 3 4 1 2 3 4 Conclusions:
Lane 1: FANCD2-Flag 293 EBNA lys.
Lane 2: 293 EBNA (-)
Lane 3: FANCD2-Flag 293 EBNA lys.
Lane 4: 293 EBNA (-)
FANCD2 
170
Conclusions:
Both antibodies work, and detect endogenous D2
Can’t resolve D2-S/D2-L in this experiment
1º ab
FANCD2-1
(B9700)
1º ab
FANCD2-2
(B9699)
1º ab FANCD2
(3686)
2º ab anti-rabbit-HRP
2º ab anti-rabbit-HRP
Alexis LaChapelle,
Hoatlin Lab

3. FANCG Antibody Test 1 2 1 2 Conclusions:
Lane 1: FANCG-HA 293 EBNA lys.
Lane 2: 293 EBNA (-)
Conclusions:
G-1 no signal (not shown)
G-2 very good and specific
NOTE: Oddly, can’t detect endogenous FANCG in 293 cells.
100 
FANCG 72 
1º ab
FANCG-2
1º ab HA
2º ab anti-rabbit-HRP
Alexis LaChapelle,
Hoatlin Lab

4. FANCF Antibody Test 1 2 1 2 Conclusions:
Lane 1: FANCF Precision Flag lys.
Lane 2: 293 EBNA (-)
FANCF 40  33 
Conclusions:
F very good and specific
1º ab
FANCF
1º ab
Flag
2º ab anti-rabbit-HRP
Alexis LaChapelle,
Hoatlin Lab