Presentation is loading. Please wait.

Presentation is loading. Please wait.

Modulation of factor VII levels by intron 7 polymorphisms: population and in vitro studies by Mirko Pinotti, Raffaella Toso, Domenico Girelli, Debora Bindini,

Published byVanessa McCoy Modified over 6 years ago

Similar presentations

Presentation on theme: "Modulation of factor VII levels by intron 7 polymorphisms: population and in vitro studies by Mirko Pinotti, Raffaella Toso, Domenico Girelli, Debora Bindini,"— Presentation transcript:

1 Modulation of factor VII levels by intron 7 polymorphisms: population and in vitro studies
by Mirko Pinotti, Raffaella Toso, Domenico Girelli, Debora Bindini, Paolo Ferraresi, Maria L. Papa, Roberto Corrocher, Giovanna Marchetti, and Francesco Bernardi Blood Volume 95(11): June 1, 2000 ©2000 by American Society of Hematology

2 Mirko Pinotti et al. Blood 2000;95:3423-3428
©2000 by American Society of Hematology

3 Mirko Pinotti et al. Blood 2000;95:3423-3428
©2000 by American Society of Hematology

4 Schematic diagram of the F7 gene and mRNA regions expressed in mammalian cells.(A) pcDNA3 plasmid (pIVS7H5, pIVS7H6, pIVS7H7, pIVS7H8, pIVS7 + 5A, and pIVS7 + 7G) containing the different allelic forms ordered by repeat number. Schematic diagram of the F7 gene and mRNA regions expressed in mammalian cells.(A) pcDNA3 plasmid (pIVS7H5, pIVS7H6, pIVS7H7, pIVS7H8, pIVS7 + 5A, and pIVS7 + 7G) containing the different allelic forms ordered by repeat number. (B) Spliced transcription products from plasmids and the size of RT-PCR–amplified fragments. pIVS7 + 5A, used as an internal control, produces the altered transcript (+5A), which contains an additional 37-bp sequence (indicated by filled square). Mirko Pinotti et al. Blood 2000;95: ©2000 by American Society of Hematology

5 mRNA expression studies with RT-PCR–amplified fragments of FVII mRNA expressed in BHK cells cotransfected with pIVS7H5 and pIVS7 + 5A (internal control).The following pIVS7 + 5A/pIVS7H5 DNA concentration ratios were used in lanes 1-4: 1:1, 1:0.3, 1:0.15, an... mRNA expression studies with RT-PCR–amplified fragments of FVII mRNA expressed in BHK cells cotransfected with pIVS7H5 and pIVS7 + 5A (internal control).The following pIVS7 + 5A/pIVS7H5 DNA concentration ratios were used in lanes 1-4: 1:1, 1:0.3, 1:0.15, and 1:0.1, respectively. Mirko Pinotti et al. Blood 2000;95: ©2000 by American Society of Hematology

6 Relative expression at the mRNA level of the differentIVS7 allelic forms.Cotransfections with pIVS7 + 5A and investigated constructs at concentration ratios of 1:0.1 (black columns) and 1:0.15 (gray columns) were carried out. Relative expression at the mRNA level of the differentIVS7 allelic forms.Cotransfections with pIVS7 + 5A and investigated constructs at concentration ratios of 1:0.1 (black columns) and 1:0.15 (gray columns) were carried out. Mirko Pinotti et al. Blood 2000;95: ©2000 by American Society of Hematology

Download ppt "Modulation of factor VII levels by intron 7 polymorphisms: population and in vitro studies by Mirko Pinotti, Raffaella Toso, Domenico Girelli, Debora Bindini,"

Similar presentations

Clinical Laboratory Analysis of Immunoglobulin Heavy Chain Variable Region Genes for Chronic Lymphocytic Leukemia Prognosis  Philippe Szankasi, David.

Clinical Laboratory Analysis of Immunoglobulin Heavy Chain Variable Region Genes for Chronic Lymphocytic Leukemia Prognosis  Philippe Szankasi, David.
Inhibition of SOX17 by MicroRNA 141 and Methylation Activates the WNT Signaling Pathway in Esophageal Cancer  Yan Jia, Yunsheng Yang, Qimin Zhan, Malcolm.

Inhibition of SOX17 by MicroRNA 141 and Methylation Activates the WNT Signaling Pathway in Esophageal Cancer  Yan Jia, Yunsheng Yang, Qimin Zhan, Malcolm.
Recurrent inversion breaking intron 1 of the factor VIII gene is a frequent cause of severe hemophilia A by Richard D. Bagnall, Naushin Waseem, Peter M.

Recurrent inversion breaking intron 1 of the factor VIII gene is a frequent cause of severe hemophilia A by Richard D. Bagnall, Naushin Waseem, Peter M.
A Common Human β Globin Splicing Mutation Modeled in Mice

A Common Human β Globin Splicing Mutation Modeled in Mice
by S. Kangsadalampai, and P.G. Board

by S. Kangsadalampai, and P.G. Board
by Nancy D. Borson, Martha Q. Lacy, and Peter J. Wettstein

by Nancy D. Borson, Martha Q. Lacy, and Peter J. Wettstein
Implications of somatic mutations in the AML1 gene in radiation-associated and therapy-related myelodysplastic syndrome/acute myeloid leukemia by Hironori.

Implications of somatic mutations in the AML1 gene in radiation-associated and therapy-related myelodysplastic syndrome/acute myeloid leukemia by Hironori.
Does the haplotype Met408-Del420, which was apparently predictive for imatinib efficacy, really exist and how strongly may it affect OCT1 activity? by.

Does the haplotype Met408-Del420, which was apparently predictive for imatinib efficacy, really exist and how strongly may it affect OCT1 activity? by.
Follicular lymphoma with a novel t(14;18) breakpoint involving the immunoglobulin heavy chain switch mu region indicates an origin from germinal center.

Follicular lymphoma with a novel t(14;18) breakpoint involving the immunoglobulin heavy chain switch mu region indicates an origin from germinal center.
by Hong Hao, Huiling Qi, and Manohar Ratnam

by Hong Hao, Huiling Qi, and Manohar Ratnam
Response: MMP-9 in platelets: maybe, maybe not

Response: MMP-9 in platelets: maybe, maybe not
Expression of Cell-Homologous Genes of Human Herpesvirus-8 in Human Immunodeficiency Virus-Negative Lymphoproliferative Diseases by Mario Luppi, Patrizia.

Expression of Cell-Homologous Genes of Human Herpesvirus-8 in Human Immunodeficiency Virus-Negative Lymphoproliferative Diseases by Mario Luppi, Patrizia.
Molecular characterization of in-frame and out-of-frame alternative splicings in coagulation factor XI pre-mRNA by Rosanna Asselta, Valeria Rimoldi, Ilaria.

Molecular characterization of in-frame and out-of-frame alternative splicings in coagulation factor XI pre-mRNA by Rosanna Asselta, Valeria Rimoldi, Ilaria.
Regulation of GM-CSF expression by the transcription factor c-Maf

Regulation of GM-CSF expression by the transcription factor c-Maf
The 5′‐terminal structure of the miRNA gene cluster miR‐23a∼27a∼24‐2.

The 5′‐terminal structure of the miRNA gene cluster miR‐23a∼27a∼24‐2.
Mutation Spectrum of the Survival of Motor Neuron 1 and Functional Analysis of Variants in Chinese Spinal Muscular Atrophy  Yu-jin Qu, Jin-li Bai, Yan-yan.

Mutation Spectrum of the Survival of Motor Neuron 1 and Functional Analysis of Variants in Chinese Spinal Muscular Atrophy  Yu-jin Qu, Jin-li Bai, Yan-yan.
A Common Genetic Polymorphism (46 C to T Substitution) in the 5′-Untranslated Region of the Coagulation Factor XII Gene Is Associated With Low Translation.

A Common Genetic Polymorphism (46 C to T Substitution) in the 5′-Untranslated Region of the Coagulation Factor XII Gene Is Associated With Low Translation.
Jacek Majewski  The American Journal of Human Genetics 

Jacek Majewski  The American Journal of Human Genetics 
Regulation of expression of murine transferrin receptor 2

Regulation of expression of murine transferrin receptor 2
Inhibition of SOX17 by MicroRNA 141 and Methylation Activates the WNT Signaling Pathway in Esophageal Cancer  Yan Jia, Yunsheng Yang, Qimin Zhan, Malcolm.

Inhibition of SOX17 by MicroRNA 141 and Methylation Activates the WNT Signaling Pathway in Esophageal Cancer  Yan Jia, Yunsheng Yang, Qimin Zhan, Malcolm.

Similar presentations

Ads by Google