1. Andy Jenkinson, EBI The DAS Protocol

2. Summary of Topics Technical overview Principles of communication Pros and cons DAS capabilities

3. DAS Architecture A client asks for data from many servers HTTP requests identically structured URLs, the same parameters Each server behaves in the same way pre-defined set of behaviours e.g. provide a sequence, provide annotations of a sequence Each server provides different data in the same format DAS-XML

4. DAS Concepts Reference object usually a sequence e.g. chromosome X or NT_025741 Annotation information attached to a location within a segment e.g. substitution at residue 326 of BRCA1

5. DAS Concepts Reference server server that provides core reference object data e.g. GRCh37 sequence data Annotation server server that provides annotations of reference objects Segment part of a reference object e.g. bases 100 to 200 of chromosome X ties together annotation and reference servers

6. Architectural Overview

7. DAS Concepts Commands A request for a certain type of data e.g. sequence, features, sources DAS Registry Catalogue of DAS sources Can be queried programmatically Validates adherence to the protocol

8. The DAS Protocol Defines 3 constraints transport layer: HTTP query format: constrained REST URLs response format: constrained XML Keyword: constrained

9. The DAS Protocol Defines 3 constraints transport layer: HTTP query format: constrained REST URLs response format: constrained XML

10. The DAS Protocol Defines 3 constraints transport layer: HTTP query format: constrained REST URLs response format: constrained XML Data transport Standard HTTP Includes compression Some additional headers, e.g. to indicate DAS version

11. The DAS Protocol Defines 3 constraints transport layer: HTTP query format: constrained REST URLs response format: constrained XML Well-defined query URLs A client can issue a command http://das.sanger.ac.uk/das/ccds_mouse/features?segment=... ^^^^^^^^^^^^^^^^^^^^^^^ ^^^ ^^^^^^^^^^ ^^^^^^^^ ^^^^^^^^^^^ site prefix das source command arguments

12. The DAS Protocol Defines 3 constraints transport layer: HTTP query format: constrained REST URLs response format: constrained XML XML format server responds with a simple XML document exon

13. Why DAS? Fast, targeted queries suitable for visual display Based on existing simple tech XML/HTTP/CGI dumb server, clever client - relatively low knowledge barrier for bioinformaticians with data to expose Scalable integrators (client software) get more data for zero cost

14. Why not DAS? One-dimensional queries query only by sequence position not by developmental stage, tissue type, etc (yet) Constrained generic format clients arent tailored to each data source possible data types are to some extent limited Not semantically rich ontology support optional

15. Commands: the basics Sequence give me the DNA sequence for a given segment of a reference object e.g. bases 100k – 200k of chromosome 15 Features give me all annotations offered by the data source that are attached to a given segment of the sequence

16. The sequence command /das/ /sequence? Parameters: segment=ID:start,end (one or more) ID of reference object Example: /das/ /sequence?segment=X:100,200 ;segment=Y:500,600

17. The sequence command Response: <SEQUENCE id="X start="100 stop="200 version="1.0> cctgagccagcagtggcaacccaatggggtccctttcca... <SEQUENCE id=Y start=500 stop=600 version="1.0> ctggacagcccggaaaatgagctcctcatctctaaccca...

18. The features command /das/ /features? Parameters: segment=ID:start,end (one or more) type=foo (zero or more) category=bar (zero or more) Example: /das/ /features?segment=X:100,200 ;segment=Y:500,600 ;type=SNP

19. The features command Response: 100 200 SNP sequencing 86.4 +...

20. Other Commands Stylesheet hints on how to render different types of feature e.g. exons as blue boxes, SNPs as red triangles /das/ /stylesheet Types lists the types of feature available /das/ /types

21. The stylesheet command red black...

22. So far…

23. Extensions Expanded for non-positional annotations e.g. annotating a gene or protein (rather than sequence) e.g. references, text mining annotations dont have start/end New data types protein 3D structures pairwise/multiple alignments interactions commands: structure/alignment/interaction

24. Metadata Can make a client that knows how to query a server and parse the response BUT something missing… which data sources are available on a server? which commands does a source support? what kind of reference objects does it know about?

25. The sources command /das/sources Lists a servers data sources For each source: text description list of capabilities (commands) list of coordinate systems (type of reference object) etc

26. DAS Registry Can get a list of sources from a server and find out how to query it BUT lots of DAS servers can only use ones we know about need a Yellow Pages (directory) location description capabilities reliability

27. DAS Registry third component of DAS catalogue of DAS sources Human interface validate, register, search, view statistics Programmatic interface http://www.dasregistry.org/das/sources http://www.dasregistry.org/das/coordinatesystem http://www.dasregistry.org/das/organism

28. SOA Registry Find Client Bind Server Publish

29. Example client behaviour

32. Links DAS Homepage http://www.biodas.org/ DAS Specification http://www.biodas.org/documents/spec-1.6.html DAS in Ensembl: http://www.ensembl.org/info/docs/das/index.html Mailing list: http://biodas.org/mailman/listinfo/das