1. Intracellular Transport Pathways

2. Three major coats function in the secretory pathway

3. Intracellular Transport Pathways
AP2
AP1, 3

4. Composition of the COPII coat

5. Structure of Sec13/31 cage
Stagg et al. Nature 2006

6. COPI coat assembly

7. COPI-coated vesicles

8. Clathrin-coated vesicles

9. Clathrin-coated vesicles

10. Clathrin-coated vesicles

11. Endocytosis requires dynamin GTPase

12. Dynamin forms a neck

13. - Priming:
Recruitment of GTP-binding protein
GEF localization!
- Coat recruitment
- Cargo recruitment
interactions with coat components
fusion machinery components

14. SNARE Hypothesis v- and t-SNAREs are part of the fusion machinery
v- and t-SNAREs provide compartment
specificity

16. “SNAREpin model

17. HA-induced membrane fusion

18. SNARE-mediated fusion

19. SNARE-mediated fusion

20. v/t SNARE fusion cycle

21. v/t SNAREs are compartment-specific
From McNew et al. (2000)

22. v/t SNAREs are compartment-specific
From Parlati et al. (2000)

23. compartment-specific
v/t SNAREs are
compartment-specific
From Parlati et al.
(2000)

24. v/t SNAREs are compartment-specific
Parlati et al. (2000)

25. Flipped SNAREs can fuse cells
Hu et al., Science 2003