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Monocyte chemoattractant chemokines in cystic fibrosis

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Presentation on theme: "Monocyte chemoattractant chemokines in cystic fibrosis"— Presentation transcript:

1 Monocyte chemoattractant chemokines in cystic fibrosis
Satish Rao, Adam K.A. Wright, William Montiero, Loems Ziegler-Heitbrock, Jonathan Grigg  Journal of Cystic Fibrosis  Volume 8, Issue 2, Pages (March 2009) DOI: /j.jcf Copyright © 2008 European Cystic Fibrosis Society. Terms and Conditions

2 Fig. 1 Determination of absolute numbers of blood monocytes by flow cytometry. Peripheral blood leucocytes are identified using flow cytometry by light scatter characteristics. Mononuclear cells are identified in gate R2 (A). Gate R3 (A) is placed around flow-count beads. Events in R2 are gated onto a CD14/HLA DR dot plot. Gate R4 (B) defines events that express HLA DR and CD14. Events in R4 are then given in the CD14 vs. CD16 dot plot (C). CD14++CD16− monocytes are represented in R6 and CD14+CD16+ monocytes in gate R5. The absolute count is derived from the number of flow-count beads represented in gate R8. Journal of Cystic Fibrosis 2009 8, DOI: ( /j.jcf ) Copyright © 2008 European Cystic Fibrosis Society. Terms and Conditions

3 Fig. 2 A) Representative histograms of CCR2 and CX3CR1 expression on CD14++CD16− blood monocytes from CF and control subjects. The shaded histogram and open histograms denote non-specific background staining and specific antibody staining respectively. Median fluorescence intensity is calculated from the difference between specific staining (open histogram) and non-specific staining (shaded). Both CCR2 and CX3CR1 are expressed on both CF and control CD14++CD16− monocytes but there was no difference between the groups. B) Representative histograms of CCR2 and CX3CR1 expression on CD14+CD16+ blood monocytes from CF and control subjects. The shaded histogram and open histograms denote non-specific background staining and specific antibody staining respectively. Median fluorescence intensity is calculated from the difference between specific staining (open histogram) and non-specific staining (shaded). CX3CR1 but not CCR2 is expressed on both CF and control CD14+CD16+ monocytes. There was no difference between the groups in CX3CR1 expression. Journal of Cystic Fibrosis 2009 8, DOI: ( /j.jcf ) Copyright © 2008 European Cystic Fibrosis Society. Terms and Conditions

4 Fig. 3 A) Dot plot showing concentrations of blood CCL2, formally called monocyte chemoattractant protein-1, in cystic fibrosis patients compared with healthy controls. The horizontal bar represents median value. ⁎p<0.01 by Mann Whitney test. B) Concentrations of CX3CL1, formally called fractalkine, in blood from cystic fibrosis patients compared with healthy controls. The horizontal bar represents median value. There is no significant difference between the groups by Mann Whitney Test. Journal of Cystic Fibrosis 2009 8, DOI: ( /j.jcf ) Copyright © 2008 European Cystic Fibrosis Society. Terms and Conditions

5 Fig. 4 A) Dot Plot showing concentrations of CCL2 in induced sputum of cystic fibrosis patients compared with healthy controls. The horizontal bar represents the median value. ⁎p<0.05 by Mann Whitney test. B) Concentrations of CX3CL1 in sputum of cystic fibrosis patients compared with healthy controls. The horizontal bar represents median value. There is no significant difference between the groups by Mann Whitney test. Journal of Cystic Fibrosis 2009 8, DOI: ( /j.jcf ) Copyright © 2008 European Cystic Fibrosis Society. Terms and Conditions

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