Presentation is loading. Please wait.

Presentation is loading. Please wait.

Monocyte chemoattractant chemokines in cystic fibrosis

Published byMyron Bell Modified over 5 years ago

Similar presentations

Presentation on theme: "Monocyte chemoattractant chemokines in cystic fibrosis"— Presentation transcript:

1 Monocyte chemoattractant chemokines in cystic fibrosis
Satish Rao, Adam K.A. Wright, William Montiero, Loems Ziegler-Heitbrock, Jonathan Grigg  Journal of Cystic Fibrosis  Volume 8, Issue 2, Pages (March 2009) DOI: /j.jcf Copyright © 2008 European Cystic Fibrosis Society. Terms and Conditions

2 Fig. 1 Determination of absolute numbers of blood monocytes by flow cytometry. Peripheral blood leucocytes are identified using flow cytometry by light scatter characteristics. Mononuclear cells are identified in gate R2 (A). Gate R3 (A) is placed around flow-count beads. Events in R2 are gated onto a CD14/HLA DR dot plot. Gate R4 (B) defines events that express HLA DR and CD14. Events in R4 are then given in the CD14 vs. CD16 dot plot (C). CD14++CD16− monocytes are represented in R6 and CD14+CD16+ monocytes in gate R5. The absolute count is derived from the number of flow-count beads represented in gate R8. Journal of Cystic Fibrosis 2009 8, DOI: ( /j.jcf ) Copyright © 2008 European Cystic Fibrosis Society. Terms and Conditions

3 Fig. 2 A) Representative histograms of CCR2 and CX3CR1 expression on CD14++CD16− blood monocytes from CF and control subjects. The shaded histogram and open histograms denote non-specific background staining and specific antibody staining respectively. Median fluorescence intensity is calculated from the difference between specific staining (open histogram) and non-specific staining (shaded). Both CCR2 and CX3CR1 are expressed on both CF and control CD14++CD16− monocytes but there was no difference between the groups. B) Representative histograms of CCR2 and CX3CR1 expression on CD14+CD16+ blood monocytes from CF and control subjects. The shaded histogram and open histograms denote non-specific background staining and specific antibody staining respectively. Median fluorescence intensity is calculated from the difference between specific staining (open histogram) and non-specific staining (shaded). CX3CR1 but not CCR2 is expressed on both CF and control CD14+CD16+ monocytes. There was no difference between the groups in CX3CR1 expression. Journal of Cystic Fibrosis 2009 8, DOI: ( /j.jcf ) Copyright © 2008 European Cystic Fibrosis Society. Terms and Conditions

4 Fig. 3 A) Dot plot showing concentrations of blood CCL2, formally called monocyte chemoattractant protein-1, in cystic fibrosis patients compared with healthy controls. The horizontal bar represents median value. ⁎p<0.01 by Mann Whitney test. B) Concentrations of CX3CL1, formally called fractalkine, in blood from cystic fibrosis patients compared with healthy controls. The horizontal bar represents median value. There is no significant difference between the groups by Mann Whitney Test. Journal of Cystic Fibrosis 2009 8, DOI: ( /j.jcf ) Copyright © 2008 European Cystic Fibrosis Society. Terms and Conditions

5 Fig. 4 A) Dot Plot showing concentrations of CCL2 in induced sputum of cystic fibrosis patients compared with healthy controls. The horizontal bar represents the median value. ⁎p<0.05 by Mann Whitney test. B) Concentrations of CX3CL1 in sputum of cystic fibrosis patients compared with healthy controls. The horizontal bar represents median value. There is no significant difference between the groups by Mann Whitney test. Journal of Cystic Fibrosis 2009 8, DOI: ( /j.jcf ) Copyright © 2008 European Cystic Fibrosis Society. Terms and Conditions

Download ppt "Monocyte chemoattractant chemokines in cystic fibrosis"

Similar presentations

Volume 94, Issue 2, Pages (March 2014)

Volume 94, Issue 2, Pages (March 2014)
CXCR6 and CCR5 Localize T Lymphocyte Subsets in Nasopharyngeal Carcinoma  Greg Parsonage, Lee Richard Machado, Jan Wai-Ying Hui, Andrew McLarnon, Tilo.

CXCR6 and CCR5 Localize T Lymphocyte Subsets in Nasopharyngeal Carcinoma  Greg Parsonage, Lee Richard Machado, Jan Wai-Ying Hui, Andrew McLarnon, Tilo.
Figure e-1A.

Figure e-1A.
Expression of FcγRIII (CD16) on human peripheral blood eosinophils increases in allergic conditions  Francis Davoine, MSc, Sophie Lavigne, MSc, Jamila.

Expression of FcγRIII (CD16) on human peripheral blood eosinophils increases in allergic conditions  Francis Davoine, MSc, Sophie Lavigne, MSc, Jamila.
Alterations in blood leukocytes of G551D-bearing cystic fibrosis patients undergoing treatment with ivacaftor  Preston E. Bratcher, Steven M. Rowe, Ginger.

Alterations in blood leukocytes of G551D-bearing cystic fibrosis patients undergoing treatment with ivacaftor  Preston E. Bratcher, Steven M. Rowe, Ginger.
Volume 61, Issue 2, Pages (February 2012)

Volume 61, Issue 2, Pages (February 2012)
Reduced Frequency of Regulatory T Cells in Peripheral Blood Stem Cell Compared to Bone Marrow Transplantations  Céline Blache, Joe-Marc Chauvin, Aude.

Reduced Frequency of Regulatory T Cells in Peripheral Blood Stem Cell Compared to Bone Marrow Transplantations  Céline Blache, Joe-Marc Chauvin, Aude.
Correlation of allergen-specific T follicular helper cell counts with specific IgE levels and efficacy of allergen immunotherapy  Yin Yao, MD, Cai-Ling.

Correlation of allergen-specific T follicular helper cell counts with specific IgE levels and efficacy of allergen immunotherapy  Yin Yao, MD, Cai-Ling.
Two cases of disseminated Mycobacterium avium infection associated with a new immunodeficiency syndrome related to CXCR4 dysfunctions  A.-V. Doncker,

Two cases of disseminated Mycobacterium avium infection associated with a new immunodeficiency syndrome related to CXCR4 dysfunctions  A.-V. Doncker,
Reduced endothelial progenitor cells in extracranial arterial stenosis but not intracranial arterial stenosis  Zhixin Huang, MD, PhD, Xiaohao Zhang, MD,

Reduced endothelial progenitor cells in extracranial arterial stenosis but not intracranial arterial stenosis  Zhixin Huang, MD, PhD, Xiaohao Zhang, MD,
Reduced TH1/TH17 CD4 T-cell numbers are associated with impaired purified protein derivative–specific cytokine responses in patients with HIV-1 infection 

Reduced TH1/TH17 CD4 T-cell numbers are associated with impaired purified protein derivative–specific cytokine responses in patients with HIV-1 infection 
Reduced levels of active GLP-1 in patients with cystic fibrosis with and without diabetes mellitus  Magnus Hillman, Leif Eriksson, Lena Mared, Karin Helgesson,

Reduced levels of active GLP-1 in patients with cystic fibrosis with and without diabetes mellitus  Magnus Hillman, Leif Eriksson, Lena Mared, Karin Helgesson,
Neil E. Alexis, Marianne S. Muhlebach, David B. Peden, Terry L. Noah 

Neil E. Alexis, Marianne S. Muhlebach, David B. Peden, Terry L. Noah 
Blood basophils from cystic fibrosis patients with allergic bronchopulmonary aspergillosis are primed and hyper-responsive to stimulation by aspergillus.

Blood basophils from cystic fibrosis patients with allergic bronchopulmonary aspergillosis are primed and hyper-responsive to stimulation by aspergillus.
Cord Blood–Derived and Peripheral Blood–Derived Cytokine-Induced Killer Cells Are Sensitive to Fas-Mediated Apoptosis  Ludovic Durrieu, Mame Massar Dieng,

Cord Blood–Derived and Peripheral Blood–Derived Cytokine-Induced Killer Cells Are Sensitive to Fas-Mediated Apoptosis  Ludovic Durrieu, Mame Massar Dieng,
Is infection with hypermutable Pseudomonas aeruginosa clinically significant?  Adi Auerbach, Eitan Kerem, Marc Victor Assous, Elie Picard, Maskit Bar-Meir 

Is infection with hypermutable Pseudomonas aeruginosa clinically significant?  Adi Auerbach, Eitan Kerem, Marc Victor Assous, Elie Picard, Maskit Bar-Meir 
Zachary M. Sellers, Lori McGlocklin, Andrea Brasch 

Zachary M. Sellers, Lori McGlocklin, Andrea Brasch 
William G. Flight, Jonathan Shaw, Susan Johnson, A

William G. Flight, Jonathan Shaw, Susan Johnson, A
Type 2 innate lymphoid cells in induced sputum from children with severe asthma  Prasad Nagakumar, MBBS, Laura Denney, PhD, Louise Fleming, MD, Andrew.

Type 2 innate lymphoid cells in induced sputum from children with severe asthma  Prasad Nagakumar, MBBS, Laura Denney, PhD, Louise Fleming, MD, Andrew.
IL-13-Stimulated Human Keratinocytes Preferentially Attract CD4+CCR4+ T cells: Possible Role in Atopic Dermatitis  Rahul Purwar, Thomas Werfel, Miriam.

IL-13-Stimulated Human Keratinocytes Preferentially Attract CD4+CCR4+ T cells: Possible Role in Atopic Dermatitis  Rahul Purwar, Thomas Werfel, Miriam.

Similar presentations

Ads by Google