1. Volume 5, Issue 6, Pages 1333-1345 (November 2012)
LATHYROIDES, Encoding a WUSCHEL-Related Homeobox1 Transcription Factor, Controls Organ Lateral Growth, and Regulates Tendril and Dorsal Petal Identities in Garden Pea (Pisum sativum L.) 
Li-Li Zhuang, Mike Ambrose, Catherine Rameau, Lin Weng, Jun Yang, Xiao-He Hu, Da Luo, Xin Li 
Molecular Plant 
Volume 5, Issue 6, Pages (November 2012)
DOI: /mp/sss067
Copyright © 2012 The Authors. All rights reserved. Terms and Conditions

2. Figure 1 Phenotypic Analysis of lath and nao1 Mutants.
(A) Front and side views of flowers and flattened petals of wild-type pea JI2822 and lath1-2 mutant. Red arrows indicate the narrow strip of dorsal petal in lath mutant compared with wild-type. DP, dorsal petal; LP, lateral petal; VP, ventral petal.
(B) Measurements of dorsal, lateral, and ventral petal size of JI2822, lath1-2, lst1-2, and lath1-2 lst1-2. This graph presents all petal size in terms of the dorsal petal size. n=12, error bars indicate ±SD.
(C) Compound leaf phenotype of JI2822 and lath1-2. Red arrows indicate the tendrils are transformed into leaflets in lath mutant compared with wild-type. Spl, stipule; FL, first pair of leaflets; SL, second pair of leaflets; FT, first pair of tendrils; ST, second pair of tendrils; TT, terminal tendril.
(D) Measurements of the first pair of leaflets length and width in JI2822 and lath1-2 mutant. n=10, error bars indicate ±SD. LL, leaflet length; LW, leaflet width.
(E) Front and side views of flowers and flattened petals of wild-type L. japonicus Gifu and nao1 mutant. Red arrows indicate the narrow strip of dorsal petal in nao1 mutant compared with wild-type. (A, C, E) Scale bar=1cm.
Molecular Plant 2012 5, DOI: ( /mp/sss067)
Copyright © 2012 The Authors. All rights reserved. Terms and Conditions

3. Figure 2
Ontology of Floral Organ and Petal Development of Wild-Type and lath Mutant.
(A) Floral organs at stage 4 in wild-type. Scale bar=50μm.
(B) Floral organs at early stage 5 in wild-type. Scale bar=50μm.
(C) Floral organs at late stage 5 in wild-type. Scale bar=100μm.
(D) Floral organs at stage 6 in wild-type. Scale bar=100μm.
(E) Floral organs at stage 7 in wild-type. Scale bar=200μm.
(F) Floral organs at stage 4 in lath mutant. Scale bar=50μm.
(G) Floral organs at early stage 5 in lath mutant. Scale bar=50μm.
(H) Floral organs at late stage 5 in lath mutant. Scale bar=100μm.
(I) Floral organs at stage 6 in lath mutant. Red arrow indicates a narrower, pointed standard petal is found in mutant. Scale bar=100μm.
(J) Floral organs at stage 7 in the lath mutant. Red arrow indicates the DP that has become more divergent from wild-type. Scale bar=200μm.
Sad, dorsal sepal; CPad, dorsal common primordium; CPl, lateral common primordium; CPab, ventral common primordium; Ca, carpel; DP, dorsal petal; LP, lateral petal; VP, ventral petal; St, stamen.
Molecular Plant 2012 5, DOI: ( /mp/sss067)
Copyright © 2012 The Authors. All rights reserved. Terms and Conditions

4. Figure 3 Comparative Genomic-Based Cloning of LATH.
(A) Syntenic analysis of LATH locus in pea, Medicago, and Lotus. The LATH locus was mapped in garden pea and found to locate on the long arm of linkage group VII. Dotted lines indicate the homologous makers or genes. Then LATH was projected to the genome of Medicago and the syntenic region was identified. NAO1 was anchored in a 250-kb contig of L. japonicus.
(B) The gene structure of NAO1. nao1 carried a C-to-T mutation. Gene structure represented as light green boxes for exons and black lines for introns. The red box indicates the homeodomain, the light blue box indicates the WUS box, and the purple red box indicates the STF box.
(C) LATH gene structure showing the position of the lath mutation site in six independent mutant lines. lath1-2 and lath1-4 are complete deletion. Black arrows show the starting sites of deletions in lath1-3, lath1-5, and lath1-6 mutants.
(D) Sequence alignment of LATH, NAO1, STF, and MAW proteins using full-length amino acid sequences. The homeodomain, WUS box, and STF box are underlined in red, light blue, and purple red, respectively.
Molecular Plant 2012 5, DOI: ( /mp/sss067)
Copyright © 2012 The Authors. All rights reserved. Terms and Conditions

5. Figure 4 LATH Expression Pattern.
(A) Expression patterns of LATH in wild-type. R, root; S, stems; L, leaves; VA, vegetative apex; IM, inflorescence meristem; FB, floral buds.
(B–G) LATH expression pattern in vegetative and floral apices by RNA in situ hybridization. Sections were hybridized with a digoxigenin-labeled antisense LATH RNA probe. Black arrows indicate the expression pattern. Scale bar=200μm. (B) LATH expression in 14-day-old vegetative shoot apex viewed in longitudinal sections. LATH is absent from the central zone of SAM (white star). (C) LATH expression in the margin of leaflets and tendrils as well as in the central narrow zone corresponding to the provascular tissue in young leaf primordia. (D) LATH is strongly expressed in lateral margins in stamens. (E, F) LATH expression in petals with the strongest expression in the lateral margin of floral organ primordium. (G) LATH expression in a carpel viewed in longitudinal section.
(H) RNA in situ hybridization in the flower using LATH sense probe as negative control. Scale bar=200μm.
Spl, stipule; l, leaflet; tl, tendril; DP, dorsal petal; LP, lateral petal; VP, ventral petal; St, stamen; Ca, carpel.
Molecular Plant 2012 5, DOI: ( /mp/sss067)
Copyright © 2012 The Authors. All rights reserved. Terms and Conditions

6. Figure 5
Interaction between LATH and LST during the Dorsal Petal Development.
(A–F) Flattened petals of wild-type and mutant flowers are shown. Scale bar=1cm.
(G) Representative epidermal cells on the adaxial side of mature petals. One of the cells in different background petals was marked with red or yellow by Adobe Photoshop CS to show dorsal and lateral representative cell type. Selected parts of the petals for SEM were shown in black rectangular box in Figure 5A, 5E, and 5G. The representative epidermal cells in ventral petal showed no difference between wild-type and mutants. Scale bar=500μm.
Molecular Plant 2012 5, DOI: ( /mp/sss067)
Copyright © 2012 The Authors. All rights reserved. Terms and Conditions

7. Figure 6
Expression Analysis of Some Transcription Factors Being Regulated by LATH.
(A) Relative expression levels of TL in the vegetative apex of wild-type and mutants.
Error bars indicate ±SD.
(B) Relative expression levels of PsMYB1 and PsMYB2 in the dorsal petal of wild-type and mutants. Error bars indicate ±SD.
(C) Relative expression levels of PsHB8 and PsAPL in the dorsal petal of wild-type and mutants. Error bars indicate ±SD.
Error bars represent SD calculated from three technical replicates done on three biological replicates.
Molecular Plant 2012 5, DOI: ( /mp/sss067)
Copyright © 2012 The Authors. All rights reserved. Terms and Conditions