1. Cell Division & Specialization

2. The Eukaryotic Cell Cycle
The eukaryotic cell cycle consists of four phases.

3. Gap 1 (G1) Phase
- cell growth

4. S Phase
-DNA replication occurs

5. Gap 2 (G2) Phase
-preparation for division -G1, S, and G2 all are part of interphase.

6. M Phase
-all of the cell’s energy is focused on division into two daughter cells (mitosis and cytokinesis )

7. Most of the time….
Most of the cell cycle is spent in Interphase, preparing for nuclear <mitosis> and cytoplasmic <cytokinesis> division so that the cell is ready to undergo the next stage as soon as new cells are needed.

9. Cyclins
-group of proteins that regulate timing of the cell cycle -help to start & stop the cycle

10. Mitosis &Cell Division

11. M Phase: Cell Division Mitosis is the division of the nucleus.
In eukaryotes, cell division occurs following interphase: it is the M phase and includes mitosis and cytokinesis.
Mitosis is the division of the nucleus.
Cytokinesis is the division of the cytoplasm.

12. Mitosis
Process in which a cell’s replicated nucleus is divided in preparation for division of the cell

13. Mitosis occurs in 4 continuous phases
Prophase Metaphase Anaphase Telophase

14. 1. Prophase
-chromatin condenses and duplicated chromosomes become visible
-centrioles move to opposite sides of nucleus and radiate the spindle
-the nucleolus disappears and nuclear envelope breaks down

16. Metaphase
-spindle fibers attach to centromeres and chromosomes line up at the equatorial plate

18. Anaphase
-the centromeres split and the paired chromatids separate -chromosomes move along the shortening spindle to opposite poles of the cell

20. Telophase
-a new nuclear membrane forms around each new group of chromosomes -spindle fibers break down - chromosomes unwind to chromatin -a nucleolus becomes visible in each daughter nucleus

22. Cytokinesis
-final phase of the cell cycle Results in two cells that are genetically identical (daughter cells)

24. Cytokinesis in animal cells
The cell membrane pinches in, creating a cleavage furrow, until the mother cell is pinched in half.

25. Cytokinesis in Plants
Cellulose & other materials are transported to the midline of the cell and a new cell wall is constructed.

26. Cytokinesis in Plants

27. End Results
The process of DNA replication, the division of the nucleus, and cytokinesis results in two new cells that are genetically identical.

28. Meiosis

29. Chromosomes
Chromosomes—the strands of DNA and protein inside the cell nucleus—are the carriers of genes.

30. Diploid Cells
Half of the chromosomes come from your male parent, and half come from your female parent, giving you two alleles for each gene.
The two sets of chromosomes are homologous, meaning that each of the chromosomes from the male parent has a corresponding chromosome from the female parent. These chromosomes have the same genes, but may have different alleles.

31. Diploid Cells
A cell that contains both sets of homologous chromosomes is diploid, meaning “two sets.” The diploid number of chromosomes is sometimes represented by the symbol 2N.
For a human the diploid number is 46 or 2N=46.

32. Haploid Cells
Some cells contain only a single set of chromosomes, and therefore a single set of genes. Such cells are haploid, meaning “one set.” They have one possible allele for each gene.
The gametes of sexually reproducing organisms are haploid.
The haploid number in humans is 23 or N=23.

33. What is Meiosis?
Meiosis is the production of sperm and egg cells in organisms that reproduce sexually. Each cell has to go through the division process twice in order for the cell to end up with half the number of chromosomes.

34. How many sets of genes do multicellular organisms inherit?
A human egg is haploid (has 23 chromosomes) and a sperm is haploid (has 23 chromosomes).
Fertilization of the egg by the sperm restores the diploid number of 46 chromosomes.

35. Meiosis contains 2 separate divisions
Meiosis I Meiosis II

36. Phases of Meiosis

37. Meiosis I
Interphase
Just prior to meiosis I, the cell undergoes a round of DNA replication during interphase.

38. Prophase I
-Chromatin condenses, the nuclear membrane breaks down, & a spindle forms.

39. Prophase I
The duplicated chromosomes pair up, forming a structure called a tetrad; they undergo a process called crossing-over.
First, the chromatids of the homologous chromosomes cross over one another.

40. Prophase I Then, the crossed sections of the chromatids are exchanged.
Crossing-over is important because it produces new combinations of alleles in the cell, increasing genetic variation.

41. Metaphase I
-paired homologous chromosomes attach to the spindle and line up

42. Anaphase I
-spindle fibers pull members of each homologous chromosome pair toward opposite ends of the cell

43. Telophase I and Cytokinesis
-a nuclear membrane forms around each cluster of chromosomes and cytokinesis follows, forming two new cells.

44. Meiosis I
The two cells produced have sets of chromosomes and alleles that are different from each other and from the diploid cell that entered meiosis I. Chromosome number has been halved.

45. Meiosis II
The two cells produced by meiosis I now enter a second meiotic division.
Unlike the first division, neither cell goes through a round of chromosome replication before entering meiosis II.

46. Prophase II
-chromosomes—each still consisting of two chromatids—become visible and the spindle forms

47. Metaphase II
-chromosomes line up in the center of each cell

48. Anaphase II
-chromatids to separate and move to opposite poles of the cell

49. Telophase II
-Nuclei reform around each group of chromosomes,the spindles break down, and cytokinesis occurs.

50. The two nuclear divisions in meiosis result in four daughter cells forming from an original parent cell, each with half the chromosomes of the parent cell.

51. Mendelian Genetics

52. Heredity The delivery of characteristics from parent to offspring is called heredity.

53. Genetics
The scientific study of heredity, known as genetics, is the key to understanding what makes each organism unique.

54. Trait
A specific characteristic or feature of an individual that may vary from one individual to another.

55. 1st Important discovery by Mendel
Law of Unit Characters -There are units in a cell that are responsible for traits (genes), and these units come in pairs (otherwise known as alleles). Mendel believed each offspring received one allele from each parent, meaning each sperm or egg carries one possible allele.

56. Genes
the unit that determines traits; it is a segment of DNA that contains information. Ex. If a plant is tall, it has a gene for being tall.

57. Alleles
Different forms of a gene that determine a specific trait

58. Multiple Allele Possibilities
Some genes have only 2 alleles while others have dozens of different alleles

59. Identifying Alleles
Alleles are represented by either an uppercase or a lowercase letter

60. 1. Dominant Allele
Allele that will always be expressed when present

61. Dominant Expressed by Uppercase Letters Ex
Dominant Expressed by Uppercase Letters Ex. Dominant allele for tall: “T”

62. 2. Recessive Allele
Allele that will be expressed only when the dominant allele for that trait is not present. Represented by lowercase letters

63. Recessive
Example: Recessive Allele for short “t”, where tall is dominant, or T

64. Law of Dominance
Mendel’s second important discovery….. Some alleles are dominant and others are recessive. An organism with at least one dominant allele for a particular form of a trait will exhibit that form of the trait. An organism with a recessive allele for a particular form of a trait will exhibit that form only when the dominant allele for the trait is not present.

65. Gamete
the sex cells -sperm or egg

66. Gametes and alleles
During gamete formation, alleles, such as tall or short, separate from each other with each gamete carrying only one allele for each gene.

67. Law of Segregation
Mendel’s 3rd discovery…. States that the alleles for a trait separate when gametes are formed. The allele pairs are then randomly united at fertilization.

68. Homozygous Alleles
Have 2 same alleles for a trait; the organism will have a pair of identical alleles—either two dominant or two recessive

69. Heterozygous Alleles
Have 2 different alleles; the organism will have one dominant & one recessive allele

70. Punnett Squares

71. Punnett Square
a chart that illustrates Mendel’s test-crosses between organisms; it is a determination of what traits may result after two parent alleles have crossed using mathematical probability.

72. Phenotype
The form of the trait that an organism displays Ex>A plant can express a phenotype for either tall or short; it may be homozygous dominant or heterozygous, both tall, or homozygous recessive, short

73. Genotype An organism’s genetic composition
It will specify the actual alleles that make up the genetic trait.

74. Monohybrid Cross a genetic cross examining ONE TRAIT
(Ex> The trait for tall vs. short)

75. R R Rr r r

76. Independent Assortment
Mendel wondered if the segregation of one pair of alleles affects another pair.
Mendel performed an experiment that followed two different genes as they passed from one generation to the next,a two-factor, or dihybrid, cross.

77. Dihybrid Cross
Cross examining inheritance of 2 traits

78. The Law of Independent Assortment
Mendel’s experimental results were very close to a 9:3:3:1 ratio.
Mendel had discovered his 4th law, the Law of Independent Assortment. The Law of Independent Assortment states that genes for different traits can segregate independently during gamete formation.

79. RrTt x rrtt
rt rt rt rt
RrTt
Rrtt
rrTt
rrtt RT Rt rT rt

80. Exceptions To The Rules

81. 1.Incomplete Dominance
Cases in which one allele is not completely dominant over another are called incomplete dominance.
In incomplete dominance, the heterozygous phenotype lies somewhere between the two homozygous phenotypes.
Third phenotype produced that is a blending of the parental traits. (2 alleles produce 3 phenotypes.)
Example: straight hair, wavy, curly
Red, pink, white flowers

82. 2. Codominance
Cases in which the phenotypes produced by both alleles are clearly expressed are called codominance.
For example, in certain varieties of chicken, the allele for black feathers is codominant with the allele for white feathers.
Heterozygous chickens have a color described as “erminette,” speckled with black and white feathers. The heterozygous organism displays both phenotypes.

83. 3. Polygenic Traits
Traits that are determined by more than one gene for a characteristic. Polygenic means “many genes.” Often show a wide range of phenotypes. Human examples: Hair, eye, and skin color

84. 4. Multiple Alleles A single gene can have many possible alleles.
A gene with more than two possible alleles is said to have multiple alleles.