1. Display-O-Matic
Think Outside the Cell

2. Passenger: Type III Needle scFv
Display-O-matic
Passenger: Type III Needle scFv
TypeIII Needle scFv: detection of and probiotic for shigella typeIII needle complex
Functional Assay:
Incubate DH10B cells with his-tagged espA
Wash and lyse cells
Dry lysate on nitrocellulose, block, and stain with anti-his antibody
Negative controls:
Results:
Displayers only:
Displayers + Needle scFv : 1 2 3 4 5 6 7 8 9
Dot blot of …legend
ehaB, VtaA11, OprF, eCPX, yuaQ, azo1653, CPG_L2, espP(beta), AIDA-1