1. Desmoplakin is a direct transcriptional target of Bcl11b.
Desmoplakin is a direct transcriptional target of Bcl11b. (A–D) Desmoplakin RNA in‐situ hybridization of hippocampal cryostat sections of control (A, C) and Bcl11bflox/flox;Emx1‐Cre (B, D) animals at P10 (A, B) and P30 (C, D). (E–H) Co‐expression analysis of Dsp (green) and Bcl11b (red) (E, G) as well as Sox2 (red) (F, H) of wild‐type dentate gyrus at P14. Images taken at × 20 (E, F) and × 63 (G, H) magnification. Scale bars: 100 μm (E); 20 μm (G). (I) Determination of hippocampal relative mRNA expression levels of Desmoplakin and Calbindin by quantitative RT–PCR at P14 (2−δδCT method; t‐test, *P<0.05; **P<0.005; error bar, s.d.; n=4). (J) Determination of direct interaction of Bcl11b and Desmoplakin by Chromatin immunoprecipitation (ChIP). ChIP assays were performed on hippocampus tissue of P14 animals employing a Bcl11b‐specific antibody (Bcl11bAB), IgG as a negative control and an RNA polymerase II (RNA Pol II AB)‐specific antibody as positive control. Direct interactions were determined by qPCR using specific primer pairs for Desmoplakin regulatory regions as well as a specific primer pair for the Gapdh promoter region (2−δδCT method; t‐test, ***P<0.001; ****P<0.0001; error bar, s.d.; hippocampi of 10 animals were used per assay).
Ruth Simon et al. EMBO J. 2012;31:
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