1. Laboratory technology for population-based screening for severe combined immunodeficiency in neonates: The winner is T-cell receptor excision circles 
Jennifer M. Puck, MD 
Journal of Allergy and Clinical Immunology 
Volume 129, Issue 3, Pages (March 2012)
DOI: /j.jaci
Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2. Fig 1
Generation of the δRec-ψJα TRECs. The germline configuration of the TCRA locus, with TCRD embedded, is shown at the top of the illustration, which also shows the points (gray dots) at which the DNA is cut to excise the TCRD locus in T-lymphocyte progenitors destined to express the α and β TCRs. After excision (lower left) and ligation (lower right) of the δRec-ψJα fragment to form a TREC, PCR primers (horizontal arrows) can amplify a DNA junction fragment containing the joint.
Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )
Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3. Fig 2
TREC (o) and actin (x) copy numbers measured in DNA isolated from 300 (A) anonymous DBSs obtained in newborn nurseries, showing one sample (far right) with failure of DNA amplification and 20 (B) newborn nursery DBSs recovered from storage from infants later found to have SCID.
Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )
Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions