1. Welcome! X-Ray Facility, 410-413 Kasha Laboratory
The presentation is intended to showcase the capabilities of the Kasha Laboratory’s Macromolecular X-Ray Diffraction Facility.
It will feature approximately twenty slides and will last one half hour.
X-Ray Facility, Kasha Laboratory
Institute of Molecular Biophysics
Florida State University
Tallahassee, FL
ã Thayumanasamy Somasundaram,

2. X-Ray Facility Orientation
History of the Facility
Facility Resources
Requirements
Data Collection
Data Processing & Manuals
Archiving & Summary
Contact & Information
This slide provides an overview of the presentation. Each item will be elaborated later in one or multiple slides.
ã Thayumanasamy Somasundaram,
11/21/2018

3. History of the Facility-1
Important Dates
Core facility planned in
Facility Director hired Feb 1993
Construction June – Sep 1993
Two generators & Image Plate (IP) detector in January 1994
Elliott generator in 1995
This slide shows the time line of the IMB X-Ray Facility. Important additions and modifications are shown.
ã Thayumanasamy Somasundaram,
11/21/2018

4. History of the Facility-2
Important Dates
Cryo capabilities in 1996
Crystal chamber in 1999
Confocal mirror in Spring 2000
CCD w/ confocal mirror in Fall 2000
CryoCooler 700 w/ auto-refill in Fall 2000
Additional Linux machines in 2000
Complete renovation during
This continuation slide shows the time line of the IMB X-Ray Facility. Important additions and modifications are shown.
ã Thayumanasamy Somasundaram,
11/21/2018

5. Facility Resources & Accessories
Three Copper rotating anode generators
Two automated area detectors
Two confocal & one total reflection mirrors
Two cryo coolers
One automated liquid Nitrogen refiller
Linux and Windows NT computers
Full time Faculty/Staff (State-line)
This slide gives the overview of the X-Ray Facility Resources
ã Thayumanasamy Somasundaram,
11/21/2018

6. Resources-1 Generators Two Rigaku H2R 5.4 kW generators
One Elliott GX kW generator
Area detector-1 (IP)
R-Axis IIc (1994) w/ confocal blue mirror
mm sample-detector distance
0-25 degree non-zero 2-theta capability
Oxford Cryostream 600 cold head
This and next slides show the core instruments of the X-Ray Facility and a brief description of their capabilities.
ã Thayumanasamy Somasundaram,
11/21/2018

7. Resources-2 Area detector-2 (CCD) Mar165 CCD w/ confocal purple mirror
mm sample-CCD distance
Limited non-zero 2-theta capability
Oxford Cryostream 700 cold head
Long & short Supper (total-reflection) mirrors
Stand-alone IP Bio Unit I Analytical Lab
This and previous slides show the core instruments of the X-Ray Facility and a brief description of their capabilities.
ã Thayumanasamy Somasundaram,
11/21/2018

8. Accessories-1 Leica stereo microscope w/ Moticam digital camera
Nikon Optiphot microscope w/ analog camera
Stereo zoom microscopes
DVD-R & +R writers
DDS-3 & -4 tape drives
FireWire (Maxtor: 60 & 80 GB) drives
Windows NT and five Linux computers
This and next slides show the auxiliary instruments & computational resources of the X-Ray Facility.
ã Thayumanasamy Somasundaram,
11/21/2018

9. Accessories-2 Cold room for 4°C crystallization set-up
Room for ambient crystallization set-up
Crystallization chamber
Temperature & humidity monitoring
Dry Cryo Shipper
Long-term sample storage
Laptop for synchrotron trips
This and previous slides show the auxiliary instruments & computational resources of the X-Ray Facility.
ã Thayumanasamy Somasundaram,
11/21/2018

10. Requirements Member of Structural Biology Program
Radiation safety training
Computer account (UNIX/LINUX/NT)
Body and/or finger radiation badge
No cost to use
Users bring consumables:
CryoLoops, Capillaries, Tape and DVD media
This slide explains who is eligible to use X-Ray Facility and the cost associated with the usage.
ã Thayumanasamy Somasundaram,
11/21/2018

11. Data Collection-1 Sample mounting decision Capillary or loop mounting?
Data collection temperature
Ambient or cryo?
If cryo, cryo protection selection
Grow or soak crystal?
Detector decision
Image plate or CCD detector?
This and next slides address the decisions the user has to make before using the X-Ray Facility.
ã Thayumanasamy Somasundaram,
11/21/2018

12. Data Collection-2 Screening or regular data collection?
Improvement or brand-new crystal?
Resolution decision
Low, moderate, or high-resolution?
2-theta angle
Cell dimension/detector distance?
This and next slides address the decisions the user has to make before using the X-Ray Facility.
ã Thayumanasamy Somasundaram,
11/21/2018

13. Data Collection Procedure
Check the schedule at:
Request time by
by phone:
Priority given for samples in the beam
Priority given to unstable samples
Flexible schedule & co-operation is valued
This slide explains the general procedure requesting time for collecting x-ray diffraction data at the Facility.
ã Thayumanasamy Somasundaram,
11/21/2018

14. Data Processing Procedure
Collect preliminary data & auto index
If successful, complete data collection
If not, modify procedure and try
Index, integrate, scale full data set
Repeat integration & scaling, if needed
Validate and compare
Archive & transfer files
This slide explains the general scheme of data processing during a typical data collection run at the Facility.
ã Thayumanasamy Somasundaram,
11/21/2018

15. Archiving Data is the responsibility of the “USER”
Available archiving resources:
DDS-3 & DDS-4 tape drives
DVD-R & +R writers
Users bring the media
Facility archives data (last resort)
Computer Resources may be helpful
This slide shows the available resources for archiving (back-up) the data collected at the Facility.
ã Thayumanasamy Somasundaram,
11/21/2018

16. Manuals & Documents Online version at Paper version in 410 KLB (XRF)
Paper version in 410 KLB (XRF)
Program authors’ website
Manufacturer’s website
IMB resource pages
Lab protocols and procedures
This slide shows the various manuals, documentation and other resources available to the user for a fruitful experience at the Facility.
ã Thayumanasamy Somasundaram,
11/21/2018

17. Summary State of the art facility Excellent scientific/support staff:
Computer Resources, Protein Expression Facility, Physical Biochem Facility, Electronics shop, & Machine shop
Keep it clean and in working order
Report problems to Soma (4-6448)
Summary of the Facility and other Resources at IMB.
ã Thayumanasamy Somasundaram,
11/21/2018

18. Contacts & Information-1
Data collection, processing, scheduling:
Thayumanasamy Somasundaram [SOMA]
Ph: | |
XRF (KLB ) |
Computer related: Mike Zawrotny
Ph: |
This and next slides show how to contact people for more information.
ã Thayumanasamy Somasundaram,
11/21/2018

19. Contacts & Information-2
Electronics:
Rick Wang
Ph: |
Machine Shop | Wood shop:
Wade Baggett or Ed Kirkland
Ph: |
This and previous slides show how to contact people for more information.
ã Thayumanasamy Somasundaram,
11/21/2018

20. IP & CCD Detectors IMB (2001)
Picture Gallery
IP and CCD detectors along with rotating anode generators in pre-renovated Institute of Molecular Biophysics Building. Circa 2001.
IP & CCD Detectors IMB (2001)
ã Thayumanasamy Somasundaram,
11/21/2018

21. IP & CCD Detectors DLC (2002)
Picture Gallery
IP and CCD detectors along with rotating anode generators in Dittmer Laboratory of Chemistry (temporary location). Circa 2002.
IP & CCD Detectors DLC (2002)
ã Thayumanasamy Somasundaram,
11/21/2018

22. IP & CCD Detectors KLB (2004)
Picture Gallery
IP and CCD detectors along with rotating anode generators in renovated Kasha Laboratory, Institute of Molecular Biophysics Building. Circa 2004.
IP & CCD Detectors KLB (2004)
ã Thayumanasamy Somasundaram,
11/21/2018

23. Crystal Room & Computer Room (2004)
Picture Gallery
Room-temperature crystal set-up room (KLB 412) and data-processing computer room (KLB 410A) in renovated Kasha Laboratory, Institute of Molecular Biophysics Building. Circa 2004.
Crystal Room & Computer Room (2004)
ã Thayumanasamy Somasundaram,
11/21/2018