1. Safety In the Science Lab
Lab Safety: Everyone Is Responsible!
Safety In the Science Lab
Rules and Symbols
Lumberton High School

2. Safety First Science is a hands-on laboratory class.
Safety in the science classroom is the #1 priority.
These safety rules must be followed at all times.

3. General Safety Guidelines
Be Responsible at All Times. No horseplay, etc.
Follow all instructions carefully.
Do not play with, touch or use the lab equipment until instructed to do so.
Verbal & written directions. If you don’t understand ask for help.
Do not do any experiments without teacher approval.
Food, drink, and gum are not allowed in the science classroom.
Lab Safety: Everyone Is Responsible!

4. General Safety Guidelines
Keep the science room clean and organized.
Notify the teacher immediately of any accidents or unsafe conditions in the science classroom!
Keep aisles clear, wash equipment, put notebooks in desks, etc.
Wash your hands with soap and water after experiments.
Lab Safety: Everyone Is Responsible!

5. Safety Symbols Eye Protection Sharp Objects Electrical Safety
Wear safety goggles when working with chemicals, flames, or heating devices.
If a chemical gets in your eye, flush in water for 15 minutes and notify the teacher.
Sharp Objects
When using knifes or other sharp objects always walk with the points facing down.
Cut away from fingers and body.
Electrical Safety
Do not place a cord where someone can trip over it.
Never use electricity around water.
Unplug all equipment before leaving the room.

6. Safety Symbols Animal Safety Heating Safety
Only handle living organisms with teacher permission.
Always treat living organisms humanely.
Wash your hands after handling animals.
Heating Safety
Tie back hair and loose clothes when working with open flames.
Never look into a container as you are heating it.

7. Safety Symbols Chemical Safety Hand Safety Plant Safety
Never touch, taste, or smell a chemical unless instructed by the teacher.
Hand Safety
If a chemical spills on your skin, notify the teacher and rinse with water for 15 minutes.
Plant Safety
Do not eat any plants in lab.

8. Safety Equipment Fire Blanket Fire Extinguisher On Fire?
To operate the fire extinguisher remember P-A-S-S
P- Pull the Pin
A-Aim the hose at the base of the fire from 5-6 feet away.
S-Squeeze the handle.
S-Sweep the hose back and forth across the fire.
On Fire?
REMEMBER: Stop, Drop, & Roll

9. What’s Wrong With This Picture?

10. What’s Wrong With This Picture?

11. What’s Wrong With This Picture?

12. What’s Wrong With These Statements?
Hal says that his teacher is solely responsible for preventing laboratory accidents.
Keshia started the lab activity before reading it through completely.
Ricardo decided to do a lab activity that he read about in a library book before the teacher came into the classroom.
Stephanie says that the safety goggles mess up her hair and give her raccoon eyes. She refuses to wear them.
Barbie and Ken accidentally break a beaker full of some chemical. Instead of risking getting in trouble they quickly clean up the mess with paper towel and throw it in the garbage.

13. What To Do In An Emergency
If there is a fire or fire alarm
Quietly get up and push in your chairs in.
Walk toward the outside classroom door.
Walk outside quickly and quietly.
Lab Safety: Everyone Is Responsible!

14. Any Questions? REMEMBER:
Safety is a Priority at Lumberton High School!

17. Used to look at microscopic specimens
Microscope 1
Used to look at microscopic specimens

18. Microscope Slide 2 Used to prepare specimen for microscopic
investigation

19. 3
Funnel
Used when pouring liquid into a small container

20. 4
Graduated Cylinder
Used to measure liquids

21. Used to hold and test small amounts of chemicals
Test Tube 5
Used to hold and test small amounts of chemicals

22. Test Tube Rack 6
Holds test tubes

23. Used for rough measuring and pouring of liquids7
Used for rough measuring and pouring of liquids
Beaker

24. Erlenmeyer Flask 8
Used for holding, mixing and heating liquids, and reduces evaporation of liquids. .

25. Cuts tissue during dissection9
Dissecting Scissors
Cuts tissue during dissection

26. Test Tube Brush 10
Cleans test tubes

27. 11
Forceps
Used to grasp small items

28. Used to add small amounts of liquid12
Pipette
Used to add small amounts of liquid

29. Used for eye protection
Safety Goggles 13
Used for eye protection

30. Digital Balance 14
Used to find mass

31. Balance (Triple Beam Balance)15
Used to find mass

32. Introduction to the Microscope
Care
Parts
Focusing
Students will have their microscopes out, for reference as we go through each of the steps. As an introduction, students will be asked what kinds of things they can do with this tool.

33. Bringing Out Your Microscope
Always carry with 2 hands, one on the arm and one on the base
Only use lens paper for cleaning the lens
Keep liquids away!
Teacher demonstrates how to hold the microscope, where the lens paper is located and how to use it. Students will be invited to turn the knobs and observe the stage as it moves up and down WITHOUT A SLIDE. Teacher will demonstrate how to store the microscope.

34. Name the parts of a microscope
Eyepiece
Body Tube
Revolving Nosepiece
Arm
Objective Lens
Stage
Stage Clips
Coarse Focus
This is the exact version of the microscope used in class. Students will be identifying the parts on the microscopes at their desks as we go along and what their functions are.
Diaphragm
Fine Focus
Light
Base

35. Getting Started Place the Slide on the Stage, under the Stage Clips
Click Nosepiece to the lowest power (shortest) Objective Lens
Look into the Eyepiece
Use the Course Focus knob to bring close to being in focus, then use the smaller Fine Focus knob.
Give students a slide from the “common things” set, each student will practice focusing and changing objectives.

36. Increasing Magnification
Change the Objective
Do NOT use the Coarse Focusing Knob
Use the Fine Focus Knob to bring the slide into focus
Have students exchange slides so they can look at different things, walk them through using the high power objective to focus slides. Emphasize not using the coarse objective during this process, as it will crack the slides.

37. Objective X Eyepiece = Total Magnification

38. Putting Your Microscope Away
Remove and return your Slides.
Click the Nosepiece to the lowest power Objective
Always carry back to the cart with 2 hands, one on the arm and one on the base
Have students exchange slides so they can look at different things, walk them through using the high power objective to focus slides. Emphasize not using the coarse objective during this process, as it will crack the slides.

39. Name the parts of a microscope
Eyepiece
Body Tube
Revolving Nosepiece
Arm
Objective Lens
Stage
Stage Clips
Coarse Focus
This is the exact version of the microscope used in class. Students will be identifying the parts on the microscopes at their desks as we go along and what their functions are.
Diaphragm
Fine Focus
Light
Base

40. Name the parts of a microscope
Eyepiece
Body Tube
Revolving Nosepiece
Arm
Objective Lens
Stage
Stage Clips
Coarse Focus
This is the exact version of the microscope used in class. Students will be identifying the parts on the microscopes at their desks as we go along and what their functions are.
Diaphragm
Fine Focus
Light
Base