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Drosophila Identification of various phenotypes.

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Presentation on theme: "Drosophila Identification of various phenotypes."— Presentation transcript:

1 Drosophila Identification of various phenotypes.
Biology 22 Drosophila Identification of various phenotypes.

2 Outline Brief description of Drosophila phenotypes and life cycle
collection of data related to gender and body color How to study keep your flies still during the identification.

3 Reference for Pictures:
Carolina Drosophila melanogster Manual ( ) Published: Carolina Biological Supply Company Based on research of Dr. Thomas Hunt Morgan

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5 The life Cycle

6 Life Cycle: Drosophila melanogaster
Eggs: contain filaments 2 molting phases “instars” Life cycle is 10 days

7 Fruity Fly Egg and Larva

8 Fruit Fly Pupa and Adult

9 1- EXPERIMENT: Identification of:
Gender Body Color

10 Wild Type Versus Mutants
Body Color Wild type: Beige Mutant: Yellow body

11 Parental phenotypes Experiment: body color
A= female: wt (beige) B=female: wt (beige) C=male: wt (beige) D=female: mutant (yellow) E=male: mutant (yellow)

12 Fruit Fly Body Color

13 Sex combs: Male Drosophila

14 Female and Male Fruit Flies

15 Abdominal segements 7 segments to female abdominal segments
5 segments to male abdominal segments Male body size is smaller than female But the “last” abdominal segment is larger in male

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17 Nap Time Triethylamine

18 How to anesthetize a fly

19 How to use the Napper

20 View the Fruit Fly

21 You will record class data
After counting the crosses record the data on the class spreadsheet

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