1. Drosophila Identification of various phenotypes.
Biology 22
Drosophila
Identification of various phenotypes.

2. Outline Brief description of Drosophila phenotypes and life cycle
collection of data related to gender and body color
How to study keep your flies still during the identification.

3. Reference for Pictures:
Carolina Drosophila melanogster Manual ( )
Published: Carolina Biological Supply Company
Based on research of Dr. Thomas Hunt Morgan

5. The life Cycle

6. Life Cycle: Drosophila melanogaster
Eggs: contain filaments
2 molting phases “instars”
Life cycle is 10 days

7. Fruity Fly Egg and Larva

8. Fruit Fly Pupa and Adult

9. 1- EXPERIMENT: Identification of:
Gender
Body Color

10. Wild Type Versus Mutants
Body Color
Wild type: Beige
Mutant: Yellow body

11. Parental phenotypes Experiment: body color
A= female: wt (beige)
B=female: wt (beige)
C=male: wt (beige)
D=female: mutant (yellow)
E=male: mutant (yellow)

12. Fruit Fly Body Color

13. Sex combs: Male Drosophila

14. Female and Male Fruit Flies

15. Abdominal segements 7 segments to female abdominal segments
5 segments to male abdominal segments
Male body size is smaller than female
But the “last” abdominal segment is larger in male

17. Nap Time Triethylamine

18. How to anesthetize a fly

19. How to use the Napper

20. View the Fruit Fly

21. You will record class data
After counting the crosses record the data on the class spreadsheet