1. Establishment of transplantable porcine tumor cell lines derived from MHC- inbred miniature swine
by Patricia S. Cho, Diana P. Lo, Krzysztof J. Wikiel, Haley C. Rowland, Rebecca C. Coburn, Isabel M. McMorrow, Jennifer G. Goodrich, J. Scott Arn, Robert A. Billiter, Stuart L. Houser, Akira Shimizu, Yong-Guang Yang, David H. Sachs, and Christene A. Huang
Blood
Volume 110(12):
December 1, 2007
©2007 by American Society of Hematology

2. Gross pathologic and histologic findings of lymphoma (PTLD) in miniature swine.
Gross pathologic and histologic findings of lymphoma (PTLD) in miniature swine. (A) Animals that developed lymphomas or PTLD typically had pronounced lymphadenopathy, as represented by animal Image was acquired using a Kodak camera (Eastman Kodak, Rochester, NY) model Easyshare Z740 with a 45.5-mm to 55-mm lens adapter. No further image processing was done. (B) Lymph node tissue harvested from these animals demonstrated destruction of normal architecture and predominance of abnormal cells as represented by animal Slides were viewed with an Olympus BX51 compound microscope (Olympus America, Melville, NY) of sections stained with hematoxylin and eosin (H&E; Hematoxylin Gill's Formulation no. 2, Fisher Diagnostics, Fair Lawn, NJ; Eosin-Y, Richard-Allan Scientific, Kalamazoo, MI) using a lens at 40× (left) and 400× (right). Images were acquired using an Olympus digital microscope camera (Olympus America) model Q-Color 3, and were processed with Adobe Photoshop CS version 8 software (Adobe Systems, San Jose, CA).
Patricia S. Cho et al. Blood 2007;110:
©2007 by American Society of Hematology

3. Gross pathologic and histologic findings of leukemia in miniature swine.
Gross pathologic and histologic findings of leukemia in miniature swine. (A) The most consistent findings of animals with leukemias were enlarged liver and spleen, which on palpation were firm and pale in color with visible lesions, as shown by animal Image was acquired using a Kodak camera (Eastman Kodak) model Easyshare Z740 with a 45.5-mm to 55-mm lens adapter. No further image processing was done. (B) Bone marrow from these animals was predominantly populated with abnormal cells, as represented by tissue from animal Slide was viewed with an Olympus BX51 compound microscope (Olympus America) of sections stained with H&E (Hematoxylin Gill's Formulation no. 2, Fisher Diagnostics; Eosin-Y, Richard-Allan Scientific) using a lens at 400×. Image was acquired using an Olympus digital microscope camera (Olympus America) model Q-Color 3, and was processed with Adobe Photoshop CS version 8 software (Adobe Systems).
Patricia S. Cho et al. Blood 2007;110:
©2007 by American Society of Hematology

4. In vitro growth of tumor cell lines.
In vitro growth of tumor cell lines. The 9 tumor cell lines display various patterns of growth and sizes in vitro. Most preferentially grow in clusters, although one line, 14736, grows as a single-cell suspension. (A) MML-12933; (B) LCL-13271; (C) ML-13381; (D) CML-14736; (E) CML-15433; (F) LCL-15446; (G) LCL-17016L; (H) LCL-17016P; (I) LCL Slides were viewed with a Nikon Eclipse TE2000-U microscope (Nikon Instruments, Melville, NY) using Nikon Plan Fluor lenses at 40× in a cell culture media. Images were acquired using a NIkon Eclipse TE2000-U camera (Nikon Instruments) and were processed with Adobe Photoshop Elements version 3.0 software (Adobe Systems).
Patricia S. Cho et al. Blood 2007;110:
©2007 by American Society of Hematology

5. Histologic findings after in vivo transfer of LCL-13271 tumor cells in NOD/SCID mice.
Histologic findings after in vivo transfer of LCL tumor cells in NOD/SCID mice. LCL injected intraperitoneally into NOD/SCID mice with tumor growth at 2 months in primary and secondary recipients. Abdominal tumor mass from NOD/SCID primary (A) and secondary (B) recipients of LCL Histologic findings were similar in morphology compared with the primary tumor (Huang et al35). Slides were viewed with an Olympus BX40 microscope (Olympus America) of sections stained with H&E medium (Hematoxylin Gill's Formulation no. 2, Fisher Diagnostics; Eosin-Y, Richard-Allan Scientific) using a lens at 400×. Images were acquired using a Hitachi charge-coupled device color camera (Hitachi Kokusai Electric America, Woodbury, NY) model HV-C20 3-CCD, and were processed with ACDSee version 4.0 software (ACD Systems International, Victoria, BC).
Patricia S. Cho et al. Blood 2007;110:
©2007 by American Society of Hematology

6. Histologic findings after in vivo transfer of CML-14736 tumor cells into miniature swine.
Histologic findings after in vivo transfer of CML tumor cells into miniature swine. CML grew after in vivo transfer to histocompatible miniature swine pretreated with TBI. Tumor growth was found at the subcutaneous injection sites (A) and in the lungs after intravenous administration (B). Immunohistochemistry of the subcutaneous injection site tissue negative staining for CD3, but positive staining for CD16 and CD172a, which was consistent with the surface phenotype of the primary tumor and cultured cells (C). Slides were veiwed with a Nikon Eclipse E800 microscope (Nikon Instruments) of sections stained with H&E (Hematoxylin Gill's Formulation no. 2, Fisher Diagnostics; Eosin-Y, Richard-Allan Scientific) using Nikon Plan Fluor lenses at 400× (A right; C left, middle, right) and 200× (B). Images were acquired using Nikon HRD060-NIK 0.6X optical coupler diagnostic instruments (Nikon Instruments) connected to a computer with SPOT-diagnostic instruments and was processed with SPOT Advanced or Basic version software for Windows (Diagnostic Instruments, Sterling Heights, MI).
Patricia S. Cho et al. Blood 2007;110:
©2007 by American Society of Hematology