1. Almost all of molecular biology techniques are
based on this paper

2. Figure 1
Slow cooling was better
2) More DNA more transformation
activity

3. Activity correlates to change in absorption of cooled DNA not warm DNA
Figure 2
DNA absorption at heated temp
DNA absorption
after cooling
Activity correlates to change
in absorption of cooled DNA not
warm DNA

4. Figure 3

5. Figure 4
Fast cooling?
1ug/ml to
20ug/ml

6. Figure 5 Salt dependent Annealing by Repressing the repulsive
Effect of phosphates
By shielding them.

7. Figure 6 Non-homologous DNA makes it Harder for homologous
ssDNA to find each other

26. TCA TC/T TGC TTC ACA ATG GTA/G CTC/T TTC/T CCA/G GAT TC
S S C F T M V L F P D TC
GAA TCC/T GGA/G AAA/G AGT/C ACC ATT
GTG AAG CAA/G ATG
E S G K S T I V K Q M

32. E S G K S T I V K Q M