1. Paper Chromatography and Spectrophotometry Analysis
Tyler Scott
Habib Ashogbon
Brian Uzomba
Marcus Stripling

2. Tara

3. Analyzing plant extracts

4. Extract A

5. Extract C

7. Most of her extract A spilled into extract C and now she had only a little bit of extract A, extract B and a mixture of extracts A and C.

9. How would she analyze the pigments present in the respective extracts?

10. Hypothesis
If extract A is analyzed and determined then the A+C mixture can be analyzed and extract C can be determined

11. Controls Positive Control Negative control:
Wavelengths between 401nm and 650nm
Negative control:
Wavelengths between 0nm and 400 nm
Wavelengths above 650nm

12. Materials A, B, and A+C sample Chromatography paper Capillary tubes
Beakers
Separation solvent
Scissors
Spectrophotometer and cuvette

13. Methods
Plant extracts A, B, and A+C were placed on chromatography paper by use of capillary tubes
15 mL of separation solvent was put in beaker
The chromatography paper was rolled into a cylinder and placed into the beaker with the solvent
The chromatography paper was allowed to sit in the beaker for 15 minutes, then air dried

14. Methods (continued)
The chromatography paper was divided into separate bands and each section cut into small pieces
Each section’s pieces were put into designated test tubes with 3mL of isopropyl alcohol and allowed to sit
The test tube solutions were tested for light absorption using a spectrophotometer at wavelengths from 400nm to 720 nm

15. Results

16. Graph of the Chlorophyll OD Levels
Results
Graph of the Chlorophyll OD Levels

17. Hypothesis was accepted
Results
Hypothesis was accepted
Chlorophyll A absorbs more light between range. The highest OD level was at a 440 wavelength for Chlorophyll A.
Chlorophyll B absorbs more light between range. The highest OD level was at a 480 wavelength for Chlorophyll B.
Chlorophyll C absorbs more light between range. The highest OD level was at a 580 wavelength For Chlorophyll C.

18. Conclusion
The longer the wavelength the lower the energy and the farther it moves away from the pencil line.