1. Transcription Regulation
Ayo Technology
Bacteria
Entropy…Yippee!!
Gellin’
Viruses
In Those Genes
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2. DAILY DOUBLE
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3. DAILY DOUBLE
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4. This is the primary carbohydrate that is used within the gel
agarose
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5. These are used in order to cut the DNA in the right area before it is inserted into the well plate
Restriction enzymes
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6. This liquid is used in order to assist in the conduction of electricity within the gel chamber
buffer
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7. This is the electrical cord that is closest to the wells where the DNA is inserted, while this is the electrical cord that is on the opposite side
Cathode, anode
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8. These are the restriction enzymes that were utilized within our lab
Eco RI, Lamb III
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9. This be what don’t stop, gets it, gets it, when it binds to mRNA in microarrays
cDNA
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10. This technique is new age, different from microarrays, and helps amplify DNA samples
Polymerase Chain Reaction (PCR)
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11. Insulin is what these can make and make if you can control the genes inside of bacteria
Bacterial plasmids
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12. Spotlights don’t do this justice, only fluorescence is used in order to use this technique
Microarray assays
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13. We’re not tired of using this technology which involves the use of radioactive probes to help identify proteins
Southern blotting
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14. Normal cell cycles are regulated by the presence of these
Growth factors
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15. In order to have an enhanceosome, these two components must come together
Activators, enhancers
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16. The transcription initiation complex assembles at this region of the eukaryotic gene
promoter
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17. Triggering the cell cycle to continue
Genes that are transcribed as a result of the RAS cell cycle are responsible for this
Triggering the cell cycle to continue
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18. An inactive p53 gene would most likely result in this
cancer
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19. These are the two life cycles that viruses undergo
Lytic, lysogenic
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20. This is the protective capsule found on the outside of a virus
capsid
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21. This is the enzyme that assists HIV and allows it to replicate
Reverse transcriptase
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22. This is the process where a virus injects its DNA into a bacteria and hydrolyzes the host’s DNA
Generalized transduction
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23. Viruses trick to host into helping it to perform these three processes
Replication, transcription, translation
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24. Conjugation, transformation, transduction
These are the three processes of DNA exchange that involve bacteria
Conjugation, transformation, transduction
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25. These are segments of DNA that move to a different location along the genome
transposons
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26. This is a form of DNA that can be transferred from one bacteria to another bacteria
plasmid
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27. Transposons can allow for this to take place
Antibiotic resistance
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28. The boundaries for transposons in bacteria are known as these
Inverted repeats
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29. A histone can be wound up into these structures within chromatin
nucleosome
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30. Histones fold DNA to form these areas
nucleosomes
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31. These can affect the structure and function of other proteins
proteosome
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32. Adding these two chemical groups can alter DNA
Methyl groups, acetyl groups
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33. Antibodies contain these two regions; one in which the genes remain the same, and the other where they are subject to change
Constant region, variable region
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34. This technology require specific primers in order for it to be successful
Polymerase Chain Reaction (PCR)
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35. This is the enzyme that helps to fill in the gaps within a sequence when utilizing bacterial plasmids
DNA ligase
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36. Transformation, ampicillin
This is the process that was used in our lab to try to give resistance to this particular compound
Transformation, ampicillin
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37. This is the study of protein structure, while this is the process of working to create transgenic organisms
Proteomics, genetic engineering
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38. Altering your particular genome would be this process, while this field of study would also need to be done to figure out the sequence of your genome
Gene therapy, bioinformatics
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