1. Gram stain
Marion Schoof

2. Background information
Gram stain important Method for differencaiation and classification of bacteria
Bacteria devided into two classes:
Gram-positive
Gram-negative

4. Staining: solution Time Process Cristal Violet, alcoholic 00:01:30
Coloring
Aqua nondest
00:00:30
Elution
Lugol´s Solution
00:03:00
00:00:20
Decoloring-solution of Gram
00:00:10
Decoloring
Safranin-Solution
00:01:00
Coloring (Counterstain)

6. Afterwards dehydration in:
Ethanol 96% (2 x 2min)
Isopropanol (1 x 2min)
Xylel (2 x 5 min)

7. Automatic staining and manual staining perfomed according to the same schedule
Elution under running water

8. Images manual staining: