1. Christopher Brunson
iGEM Brainstorming 2

2. Proposal
Digest completely
E. Coli
Crude Oil
convert
Collect

3. Proposal cont. Eliminate completely Digest convert
Lower-order carbon compounds
Collect
Store in vesicles and then collect

4. Proposal cont. Eliminate completely
Isolate gene from bioremediation microbes
Lower-order carbon compounds
Convert to H2 gas, burn in air to create water;
Elemental carbon
Store in vesicles and then collect
Collect bacteria using magnetosome, later have them release the crude oil or convert to ethanol, butanol, or other derivatives

5. Proposal Molecular Breeding (carotenoid paper)
Can degrade aromatic hydrocarbons
Molecular Breeding (carotenoid paper)
Deinococcus radiodurans
Can digest toluene, xylene, benzene, styrene
Modify to produce ethanol or other products

6. Clotting

7. Proposal Hemophilia Traumatic Wounds E. Coli Clotting
EPO or steroid sensing

8. Proposal cont. Hemophilia Artificial clotting factor, thrombin
Isolate gene from human DNA
Traumatic Wounds
Initiate widespread clotting by stopping blood flow
Programmed cell death once blood flow has stopped
EPO or steroid sensing
Make sensitive to EPO, steroid, or drug of choice
Change pH or color of blood

9. Proposal Factor VIII transcript variant 2 Isolate gene from human DNA
Non-glycosylated proteins, thrombin
Activated Factor IX
Factor XII (serine protease), or other missing necessary proteases
Activated Factor XI
Programmed cell death once blood flow has stopped
Use biofilm to stop bleeding
Bacterial Overload
Change pH or color of blood
Sense glycosylation residues of EPO

10. Clotting Cascade in Hemophilia
Deficiencies in necessary cofactors
As shown above, can either provide the cofactors necessary or activate the target proteins
Ex: Factor VIII is the cofactor for Factor IX; can either supply Factor VIII or activate Factor IX