1. Koshi high school Erina Mizutani Shiori Endo
Study of plant dyes
Koshi high school
Erina Mizutani
Shiori Endo

4. antibacterial properties
Dyeing cloth with foods which have antibacterial properties.
Does this cloth also have antibacterial properties?
food

5. Test 1
Purpose

6. onion

7. umeboshi

8. azuki beans

9. grape juice

10. coffee

11. honey

12. Karashi (mustard)

13. wasabi

14. Do you know umeboshi？
traditional Japanese food
very sour!
with rice!

15. Method
5%Alum

16. onion
grape

17. PDA
Culture
Plate
P・・・potato
D・・・dextrose
A・・・agar

18. Result

19. onion
AP ×
Color ×

20. umeboshi
AP ○
Color ○

21. AP ○
Color ○
azuki beans

22. grape
AP ×
Color ×

23. coffee
AP ×
Color ×

24. honey
AP ×
Color ×

25. karashi
AP ×
Color ×

26. wasabi
AP ×
Color ×

27. antibacterial properties
color
onion ×
umeboshi ○
azuki beans
grape
coffee
honey
karashi
wasabi

28. Consideration
We think the antibacterial properties are involved in the color change.

29. Test 2
Purpose
We　extracted Umeboshi’s color.

30. Method ①Mash Umeboshi into a paste. ②Put the samples in
ethanol, methanol, hexane, and
chloroform.
③Shake them.
Umeboshi paste

31. Result Solvent ↓ red Solvent ↓ yellow methanol ethanol Solvent ↓
No change
　　　 chloroform
　　　　hexane

32. Consideration
・Ethanol has a high polarity. ・We can analyze the extracted color with chromatography.

33. Summary
①The cloth dyed with Umeboshi, Azuki beans, and Wasabi have antibacterial properties. ②We think the antibacterial properties are involved in the color change. ③Ethanol is good at extracting Umeboshi’s color.

34. Issues
・We have to analyze umeboshi’s color, making sure to single out that color, and reveal a relationship between its color and its antibacterial properties. ・We have to extract and analyze the color from “red shiso”, and reveal that it also has
antibacterial properties
color

35. We think・・・ The cloths don’t have antibacterial properties.
The cloths have antibacterial properties.
germ detector
germ detector
antibacterial properties

36. Bibliography
「草木染めに挑戦しよう！」
「カロリーSlism」
「キノコ栽培サイト」

37. Thank you very much for your kind attention!!!