1. Restriction Enzyme Analysis of Lambda DNA
DNA Fingerprinting of lambda DNA

2. Purpose 1. See the DNA 2. Become familiar with -Restriction enzymes
-Electrophoresis
-Analysis of DNA bands

3. Applications of the procedure
Used to identify bacteria and viruses based on the DNA finger printing of these organisms.
Genetic screening – electrophoresis is the first step.
Forensic medicine – electrophoresis is used in DNA finger printing.

4. Lambda Phage infects E.coli.
Its DNA is about 49,000 base pairs long

7. Restrictions enzymes are molecular scissors
Recognize specific sequence cut DNA
make cuts in the DNA
Each enzyme recognizes different sequence.

8. Gel is porous
DNA fragments migrate through the gel
Electric current push the DNA
DNA is negatively charged because of the phosphates
Migrate from negative to positive
Smaller fragments migrate faster
Each band has many copies of a fragment.

10. Analysis of Lambda DNA 1 2 3 4 5 6 7 8 1,5 – Uncut 2,6 – PstI
3,7 – EcoRI
4,8 - HindIII