1. Effects of bretylium or 6-OHDA on action potential-evoked Ca2+ transients in axon bundles and varicose nerve terminals in mouse vas deferens

2. Bretylium-resistant varicosities Mouse vas deferens
Action potential-evoked calcium transients in strings of varicosities not blocked by bretylium (20 µM; 2 hours)
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3. Bretylium-sensitive varicosities
Action potential-evoked calcium transients in a string of varicosities blocked by bretylium (20 µM; 2 hours; left panel and, reversed by amphetamine (right panel). Arrows point to the string of bretylium-sensitive/amphetamine-sensitive varicosities.
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4. Lack of effect of 6-OHDA on Ca2+ transients in
preterminal axon bundles
Single pulse
10 pulses at 10 Hz
Note the vigorous contraction in the right hand panel
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