1. IL-10 reduces apoptosis and extracellular matrix degradation after injurious compression of mature articular cartilage 
P. Behrendt, A. Preusse-Prange, T. Klüter, M. Haake, B. Rolauffs, A.J. Grodzinsky, S. Lippross, B. Kurz 
Osteoarthritis and Cartilage 
Volume 24, Issue 11, Pages (November 2016)
DOI: /j.joca
Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions

2. Fig. 1
Cells with normal nuclei and NB in mature bovine articular cartilage explants. The left figure (A) shows a histological section of untreated cartilage with normally structured cell nuclei (see also insertion for higher magnification). The right figure (B) shows injured cartilage with an example of an apoptotic cell with NB (see also insert). (C) Statistical analysis of chondrocytes cultured under normal conditions and after a single load compression (strain 50%, velocity 2 mm/s) w/o supplemented IL-10 [10 ng/ml]. The level of cells with NB indicates that IL-10 has an anti-apoptotic effect on mechanically induced cell death in articular cartilage explants. a, b indicate clusters of experimental groups which are significantly different from each other with P < 0.05 (detailed P-values are given in the text). Mean values ± 95% CI (n = 5).
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions

3. Fig. 2
IL-10 reduces glycosaminoglycan release from cartilage explants after mechanical injury. GAG release was measured in supernatants of mature bovine articular cartilage explant cultures under normal culture conditions (control), the influence of IL-10, or after a single load compression (Comp; strain 50%, velocity 2 mm/s). Figure (A) displays results of an IL-10 concentration series ranging from 1 pg/ml to 20 ng/ml (n = 3). Further repetitions were performed with IL-10 [10 ng/ml] (n = 8) (B). a–d indicate clusters of experimental groups which are significantly different from each other with P < 0.05 (detailed P-values are given in the text). All mean ± 95% CI.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions

4. Fig. 3
NO release (A) and NOS2 mRNA expression (B) in mature bovine articular cartilage explants. Explants were cultured under normal culture conditions (control), the influence of IL-10 [10 ng/ml], or after a single load compression (load; strain 50%, velocity 2 mm/s). NO release was photometrically measured by Griess reagent in the culture supernatant (n = 5 animals, 30 disks/group). For mRNA expression analysis eight disks from the same animal were pooled for mRNA extraction (n = 5). Gene expression levels were normalized to that of GAPDH house-keeping gene and then normalized to the non-stimulated control group without compression, which had an expression level = 1. a, b indicate clusters of experimental groups which are significantly different from each other with P < 0.05 (detailed P-values are given in the text). All mean ± 95% CI.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions

5. Fig. 4
The effect of injurious compression on matrix degrading gene expression and pretreatment with IL-10. Levels of matrix-degrading enzymes were measured 3 days after a single compression (strain rate 2 mm/s; strain 50%) and/or incubation with IL-10 [10 ng/ml]. For each condition eight disks from the same animal were pooled for mRNA extraction (n = 5). (A) MMP-3 mRNA levels, (B) MMP-13 mRNA levels and (C) ADAMTS-4 mRNA levels. Gene expression levels were normalized to that of GAPDH house-keeping gene and then normalized to the non-stimulated control group without compression, which had an expression level = 1. a, b indicate clusters of experimental groups which are significantly different from each other with P < 0.05 (detailed P-values are given in the text). Data are presented as mean ± 95% CI.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions