1. Volume 137, Issue 1, Pages 15-17 (July 2009)
OLFM4 Is a Robust Marker for Stem Cells in Human Intestine and Marks a Subset of Colorectal Cancer Cells 
Laurens G. van der Flier, Andrea Haegebarth, Daniel E. Stange, Marc van de Wetering, Hans Clevers 
Gastroenterology 
Volume 137, Issue 1, Pages (July 2009)
DOI: /j.gastro
Copyright © 2009 AGA Institute Terms and Conditions

2. Figure 1
OLFM4 marks crypt base columnar cells. (A) In situ hybridization for OLFM4 reveals a crypt base columnar-restricted expression pattern in human small intestinal epithelium. Neither the surrounding postmitotic Paneth cells at the crypt base, nor the TA cells, reveal a positive signal. (B) Comparable stem cell-restricted expression patterns are observed with Olfm4 in situ hybridization in the small intestinal epithelium of the mouse. (C) In situ hybridization for OLFM4 in the human colon also reveals a crypt base columnar-restricted expression pattern. (D) In situ hybridization in colorectal carcinoma indicates very strong OLFM4 expression in a subset of cells within the tumor. Insert: The staining observed in wild-type crypt base columnar cells (arrow) is compared with the tumor expression (*) much weaker. (E) β-Catenin staining on a serial section of tissue used in D identifies the exact location of the tumor. Size bars represent 100 μm.
Gastroenterology  , 15-17DOI: ( /j.gastro )
Copyright © 2009 AGA Institute Terms and Conditions