Presentation is loading. Please wait.

Presentation is loading. Please wait.

Martin Wolf, PhD, Lorenz Aglas, PhD, Teresa E

Published byBrittany Anthony Modified over 5 years ago

Similar presentations

Presentation on theme: "Martin Wolf, PhD, Lorenz Aglas, PhD, Teresa E"— Presentation transcript:

1 Endolysosomal protease susceptibility of Amb a 1 as a determinant of allergenicity 
Martin Wolf, PhD, Lorenz Aglas, PhD, Teresa E. Twaroch, PhD, Markus Steiner, PhD, Sara Huber, MSc, Michael Hauser, PhD, Heidi Hofer, PhD, Maria A. Parigiani, MSc, Christof Ebner, MD, Barbara Bohle, PhD, Peter Briza, PhD, Angela Neubauer, PhD, Frank Stolz, PhD, Michael Wallner, PhD, Fatima Ferreira, DDSc, PhD  Journal of Allergy and Clinical Immunology  Volume 141, Issue 4, Pages e5 (April 2018) DOI: /j.jaci Copyright © 2017 The Authors Terms and Conditions

2 Fig 1 A, Scheme of mouse immunizations. Mice (5/group) were immunized with antigen adsorbed to ALUM (+) or with antigen alone (−). B, IgG1, IgG2a, and IgE titers were determined by ELISA. Statistical analyses were performed with ANOVA. P < .05 was considered significant. Adjusted P values are shown. C, Functional IgE level was measured via mediator release of RBL cells using sera from individual mice collected at day 28. D, Statistical analysis of the amount of protein needed to induce half-maximal mediator release in rat basophilic leukemia (RBL) cells. E, Mean titration curves of pooled sera from mice immunized with Amb a 1 and DC-PelC, with and without ALUM. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2017 The Authors Terms and Conditions

3 Fig 2 SDS-PAGE analyses of protein degradation with endolysosomal proteases (upper panels) and mass spectrometric analyses of peptides generated after 48 hours (lower panels) for (A) Amb a 1.01 and (B) DC-pelC. C, Model for the influence of antigen proteolytic stability on the intensity (as determined by antibody titers) of the immune responses, and the TH1/TH2 ratios (as determined by IgG2a/IgG1 titers in mice). “+” and “−” indicate ALUM-adsorbed and free antigen, respectively. Data of stability to endolysosomal proteolysis for Bet v 1 and for Bet-mut4, a fold-stability variant of Bet v 1, were previously published by Wallner et al9 and Machado et al,10 respectively. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2017 The Authors Terms and Conditions

4 Fig E1 A, SDS-PAGE of purified proteins. Weak bands observed at 24 and 15 kDa correspond to known degradation products of Amb a 1, designated alpha and beta chain, respectively. B, Mass spectrometry analysis of tryptic digests of purified natural Amb a 1.01 isoform and recombinant DC-pelC. C, Purity of the protein preparations, as determined by mass spectrometry. Total ion count (TIC) current of diagnostic tryptic peptides was calculated in relation to all identified peptides. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2017 The Authors Terms and Conditions

5 Fig E2 A, Circular dichroism (CD) spectra of Amb a 1.01 and DC-pelC measured at 20°C and 95°C, respectively. B, Second derivative of FTIR spectra of Amb a 1.01 and DC-pelC in the area of α-helix (1651 cm−1) and β-sheet (1637 cm−1) signals. C, Secondary structure elements as calculated from FTIR spectra. D, Structural model of Amb a 1.01 generated by Swissmodel (template: PDB:1PXZ) and DC-pelC structure (PDB:2EWE). Lower panels, structural alignment of Amb a 1.01 model and DC-pelC structure generated with the UCSF Chimera package. FTIR, Fourier-transform infrared. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2017 The Authors Terms and Conditions

6 Fig E3 IgE, total IgG, IgG1, and IgG4 ELISA experiments performed with sera from 39 ragweed pollen-allergic patients. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2017 The Authors Terms and Conditions

Download ppt "Martin Wolf, PhD, Lorenz Aglas, PhD, Teresa E"

Similar presentations

WHAT SHAPES AN ALLERGEN?

WHAT SHAPES AN ALLERGEN?
Usefulness of recombinant γ-gliadin 1 for identifying patients with celiac disease and monitoring adherence to a gluten-free diet  Bharani Srinivasan,

Usefulness of recombinant γ-gliadin 1 for identifying patients with celiac disease and monitoring adherence to a gluten-free diet  Bharani Srinivasan,
Repeated low-dose intradermal allergen injection suppresses allergen-induced cutaneous late responses  Giuseppina Rotiroti, MD, Mohamed Shamji, PhD, Stephen.

Repeated low-dose intradermal allergen injection suppresses allergen-induced cutaneous late responses  Giuseppina Rotiroti, MD, Mohamed Shamji, PhD, Stephen.
Comparison of the immunogenicity of BM32, a recombinant hypoallergenic B cell epitope–based grass pollen allergy vaccine with allergen extract–based vaccines 

Comparison of the immunogenicity of BM32, a recombinant hypoallergenic B cell epitope–based grass pollen allergy vaccine with allergen extract–based vaccines 
Birch pollen–related food allergy: Clinical aspects and the role of allergen-specific IgE and IgG4 antibodies  Marija Geroldinger-Simic, MD, Thomas Zelniker,

Birch pollen–related food allergy: Clinical aspects and the role of allergen-specific IgE and IgG4 antibodies  Marija Geroldinger-Simic, MD, Thomas Zelniker,
Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut  Claudia.

Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut  Claudia.
Kuan-Wei Chen, PhD, Katharina Blatt, MSc, Wayne R

Kuan-Wei Chen, PhD, Katharina Blatt, MSc, Wayne R
Luise Westernberg, PhD, Véronique Schulten, PhD, Jason A

Luise Westernberg, PhD, Véronique Schulten, PhD, Jason A
Identification and isolation of a Fel d 1–like molecule as a major rabbit allergen  Christiane Hilger, PhD, Stéphanie Kler, MSc, Karthik Arumugam, PhD,

Identification and isolation of a Fel d 1–like molecule as a major rabbit allergen  Christiane Hilger, PhD, Stéphanie Kler, MSc, Karthik Arumugam, PhD,
Exploring the repertoire of IgE-binding self-antigens associated with atopic eczema  Sabine Zeller, MSc, Claudio Rhyner, PhD, Norbert Meyer, MD, Peter.

Exploring the repertoire of IgE-binding self-antigens associated with atopic eczema  Sabine Zeller, MSc, Claudio Rhyner, PhD, Norbert Meyer, MD, Peter.
Identification and gene cloning of a new major allergen Cha o 3 from Chamaecyparis obtusa (Japanese cypress) pollen  Toshihiro Osada, MSc, Takafumi Harada,

Identification and gene cloning of a new major allergen Cha o 3 from Chamaecyparis obtusa (Japanese cypress) pollen  Toshihiro Osada, MSc, Takafumi Harada,
Designing hypoallergenic derivatives for allergy treatment by means of in silico mutation and screening  Theresa Thalhamer, PhD, Heidi Dobias, MSc, Tatjana.

Designing hypoallergenic derivatives for allergy treatment by means of in silico mutation and screening  Theresa Thalhamer, PhD, Heidi Dobias, MSc, Tatjana.
Odelya E. Pagovich, MD, Bo Wang, MD, Maria J

Odelya E. Pagovich, MD, Bo Wang, MD, Maria J
Frequent occurrence of T cell–mediated late reactions revealed by atopy patch testing with hypoallergenic rBet v 1 fragments  Raffaela Campana, PhD, Katharina.

Frequent occurrence of T cell–mediated late reactions revealed by atopy patch testing with hypoallergenic rBet v 1 fragments  Raffaela Campana, PhD, Katharina.
Making the Most of In Vitro Tests to Diagnose Food Allergy

Making the Most of In Vitro Tests to Diagnose Food Allergy
Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut  Claudia.

Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut  Claudia.
IgE cross-reactivity between the major peanut allergen Ara h 2 and the nonhomologous allergens Ara h 1 and Ara h 3  Merima Bublin, PhD, Maria Kostadinova,

IgE cross-reactivity between the major peanut allergen Ara h 2 and the nonhomologous allergens Ara h 1 and Ara h 3  Merima Bublin, PhD, Maria Kostadinova,
Development and characterization of a recombinant, hypoallergenic, peptide-based vaccine for grass pollen allergy  Margarete Focke-Tejkl, PhD, Milena.

Development and characterization of a recombinant, hypoallergenic, peptide-based vaccine for grass pollen allergy  Margarete Focke-Tejkl, PhD, Milena.
Blocking antibodies induced by immunization with a hypoallergenic parvalbumin mutant reduce allergic symptoms in a mouse model of fish allergy  Raphaela.

Blocking antibodies induced by immunization with a hypoallergenic parvalbumin mutant reduce allergic symptoms in a mouse model of fish allergy  Raphaela.
Oral immunotherapy induces IgG antibodies that act through FcγRIIb to suppress IgE- mediated hypersensitivity  Oliver T. Burton, PhD, Stephanie L. Logsdon,

Oral immunotherapy induces IgG antibodies that act through FcγRIIb to suppress IgE- mediated hypersensitivity  Oliver T. Burton, PhD, Stephanie L. Logsdon,

Similar presentations

Ads by Google