1. Supplementary Figure S12
A. p68 conditional knockout strategy: Provided by TaconicArtemis GmbH (not drawn to scale)
Mouse Genomic Locus
Targeting Vector
SA ~3.0 kb
LA ~6.0 kb
Neo
Targeted Allele
(after homologous
recombination)
Constitutive KO Allele
(after Cre-mediated Tk 1 13
FA ~4.0 kb
2-11
untranslated
loxP
Conditional KO Allele
(after Flp-mediated
p68 exons
frameshift 12
Puro
floxed exons
FRT F3
SA: short arm
FA: floxed arm
LA: long arm A B C
Primers (A,B,C) for PCR
B. PCR to confirm correct Cre-mediated excision of p68 gene in liver (L)
and pancreas (P) of p68loxP/loxP;ESR-Cre+ mice upon Tamoxifen treatment
A/B
A/C
PCR primers
Tamoxifen - - M
(bp) + + - - + +
Tissues L P L P L P L P
500
400
300
200